Plant Cuticle

The plant cuticle is a hydrophobic, noncellular layer covering the aerial surfaces of most land plants, where it helps regulate water loss and protects underlying tissues. It consists mainly of cutin, a polyester matrix, and embedded or deposited waxes; together, these materials limit diffusion across the epidermis while allowing controlled gas exchange through stomata. Cuticle thickness and composition vary with species and environmental conditions, influencing drought tolerance, pathogen defense, surface wettability, and interactions with insects. Studying the plant cuticle supports research in plant physiology, crop improvement, ecology, and the development of biodegradable or bioinspired barrier materials.

Plant Cuticle - Related Videos

Research

JoVE Journal - Biology

Use of Arabidopsis eceriferum Mutants to Explore Plant Cuticle Biosynthesis

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Cited by 11 •

2008

The plant cuticle is a waxy outer covering on plants that has a primary role in water conservation but is also an important barrier against the entry of pathogenic microorganisms. In this video, we demonstrate the analysis of plant cuticle mutants identified by forward and reverse genetics approaches.

Research

JoVE Journal - Biology
Free Sample

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

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Cited by 32 •

2015

Lipid polyesters constitute the structural components of two cell wall modifications, the plant cuticle and suberin-containing diffusion barriers. In this video, we describe a method to depolymerize cutin from whole delipidated leaves. The method can be applied to investigating mutants compromised in either cutin or suberin biosynthesis.

Research

JoVE Journal - Biology
Free Sample

Isolation and Biophysical Study of Fruit Cuticles

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Cited by 16 •

2012

Aerial plant organs are protected by the cuticle, a supramolecular biopolyester-wax assembly. We present protocols to monitor selective removal of epi- and intracuticular waxes from tomato fruit cuticles on molecular and micro scales by solid-state NMR and atomic force microscopy, respectively, and to assess the cross-linking capacity of engineered cuticular biopolyesters.

Cuticle Disruption: A Method to Collect Hemolymph from Drosophila Larvae

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2023

The Drosophila hemolymph serves as both blood and interstitial fluid, circulating throughout the body during the larva and adult stages. This video describes a protocol to collect larval hemolymph in order to quantify the circulating cells.

Education

JoVE Lab Manual - Biology

Plant Diversity - Concepts

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2019

From Water to Land Kingdom Plantae first appeared about 410 million years ago as green algae transitioned from water to land. Though challenging, this transition benefited early colonizers in several ways. Initially, most living organisms (including plants and animals) were ocean dwelling, making aquatic environments crowded and highly competitive. In contrast, land was a relatively uncolonized environment with ample resources and little to no predators or competitors. Terrestrial environments...

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