Plant Root Symbiosis

Plant root symbiosis is a mutually beneficial or context-dependent association between plant roots and microorganisms, and it strongly influences nutrient acquisition, growth, and ecosystem productivity. In mycorrhizal partnerships, fungal hyphae extend beyond the root, increasing access to phosphorus and water, while plants supply the fungi with carbon; in legume-rhizobium symbiosis, bacteria form root nodules and convert atmospheric nitrogen into forms plants can use. These interactions also affect soil structure, stress tolerance, and plant health. Understanding root symbiosis supports research in plant biology, sustainable agriculture, soil restoration, and strategies that reduce dependence on synthetic fertilizers.

Plant Root Symbiosis - Related Videos

Research

JoVE Journal - Biology
Free Sample

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

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Cited by 24 •

2017

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

Research

JoVE EoE - Bacterial Growth and Techniques

Treatment of Bacteria with Plant Root Exudates to Emulate In Vitro Plant-Microbe Interactions

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2025

Source: Yi, Y., et al. Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates. J. Vis. Exp. (2018)This video demonstrates the preparation and treatment of Bacillus mycoides cultures to study plant-microbe interactions. It outlines the steps from streaking a stock culture and incubating it in liquid medium to exposing the bacteria to plant root exudates under agitation. The process culminates in harvesting and flash-freezing the bacterial cells for...

Research

JoVE Journal - Biology
Free Sample

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

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Cited by 7 •

2011

We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.

Microscopy-Based Evaluation of Bacterial Attachment to Plant Root Surfaces

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2026

Source: Matthysse, A. G. Adherence of Bacteria to Plant Surfaces Measured in the Laboratory. J. Vis. Exp. (2018).This video demonstrates the evaluation of bacterial attachment to plant roots using phase-contrast microscopy. Root samples from seedlings incubated with bacteria are first mounted to check for free bacteria. The samples are then washed to remove loosely attached bacteria and remounted using a suitable coverslip to prevent damage. Finally, the number of bacteria attached to the root...

Education

JoVE Core - Biology

Symbiosis

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2026

Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...

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