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Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel
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Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

DOI:
10.3791/55044-v

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06:41 min

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January 18, 2017

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, ,
1Developmental and Cell Biology of Plants,Institute of Science and Technology Austria, 2Bioimaging Facility,Institute of Science and Technology Austria

Chapters

  • 00:00Title
  • 01:24Plant Cultivation (Arabidopsis thaliana seedlings)
  • 02:04Plant Sample Preparation
  • 03:28Microscope Setup
  • 05:18Results
  • 06:00Conclusion

Summary

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Automatic Translation

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

Tags

Keywords: Light Sheet Fluorescence MicroscopyPlant RootsGelArabidopsisSample PreparationGrowing ConditionsImaging TechniquePlant DevelopmentLiquid MediumLighting SystemLateral Roots3D PrintingSample HolderAgaroseStereo Microscope

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