Plastic Tubing Preparation
Plastic Tubing Preparation - Related Videos
13:04
Preparing and Administering Enteric Tube Medications
0 Views •
2017
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT An enteric tube is a tube that is inserted and passed into the stomach or intestines. Enteric tubes serve multiple purposes, including stomach decompression (through the removal of air, gastric contents, and secretions), enteric feeding, and/or the administration of medications or oral contrast. Enteric tubes are indicated for patients with impaired swallowing and for patients with...
03:41
Preparing Cerebral Endothelial Tubes from Mouse Parenchymal Arterioles
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2025
This video demonstrates a method for isolating the endothelial tubes from mouse cerebral parenchyma. Through a combination of enzymatic digestion and mechanical dissociation, the process yields an intact tube with endothelial cells.
10:12
Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis
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Cited by 10 •
2017
Microplastics act as vector of potentially toxic organic contaminants with unpredictable effects. This protocol describes an alternative methodology for assessing the levels of organochlorine pesticides adsorbed on plastic pellets and identifying the polymer chemical structure. The focus is on pressurized fluid extraction and attenuated total reflectance Fourier transform infrared spectroscopy.
10:56
Tube Thoracostomy
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2023
Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA Tube thoracostomy (chest tube placement) is a procedure during which a hollow tube is inserted into the thoracic cavity for drainage of fluid or air. Emergency chest tube insertion is performed for definitive treatment of tension pneumothorax, traumatic hemothorax, large-volume pleural effusions, and empyemas. Irrespective of the cause of air and fluid accumulation in the pleural space, the...
04:41
Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
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Cited by 74 •
2010
One feature of Alzheimer's Disease is the elevation of Aβ1-42 peptide. Here we provide a protocol for preparing synthetic Aβ1-42 oligomers, which impairs hippocampal Long-Term Potentiation, a cellular correlate of memory. This procedure is useful for investigating mechanisms of Aβ-induced pathology and drug screening.
FAQs
Selection should begin with the fluid, operating pressure, connector type, and sterility requirement, rather than with tubing availability alone. The polymer and internal diameter must suit those conditions so the finished line behaves consistently during transfer. This matching is especially important when an experiment depends on controlled media delivery or stable perfusion conditions.
Internal diameter influences how smoothly fluids or gases move through the assembled line and whether the transfer matches the intended experimental conditions. A mismatch can contribute to flow disturbances or unreliable delivery, even when the tubing remains connected. Choosing the diameter alongside pressure and connector requirements helps protect reproducibility in biological workflows.
Cleaning and sterility requirements determine whether prepared tubing is appropriate for handling biological materials or supporting cell culture. Cutting and assembly also need to produce smooth, secure connections, because poorly formed junctions can increase leakage, contamination, or disturbance during transfer. These factors affect both sample protection and the reliability of experimental conditions.
A typical preparation sequence consists of selecting suitable tubing, cutting it to the needed length, cleaning it according to the experiment’s requirements, and assembling it with compatible connectors. Each step supports the next: appropriate selection establishes compatibility, accurate cutting supports assembly, and clean, secure connections help maintain controlled transfer conditions.
Leak and flow problems are reduced when the tubing is cut cleanly, paired with suitable connectors, and assembled into secure, smooth junctions. The selected material and internal diameter must also fit the fluid and pressure conditions. Together, these choices support steadier transfer, reduce disruption to the setup, and help preserve experimental consistency.
Prepared tubing supports several biology workflows, including media delivery, sample handling, perfusion systems, and cell culture. In each case, consistent preparation helps maintain the intended transfer conditions while protecting samples and equipment. Reliable tubing assemblies therefore contribute to reproducibility when liquids or gases must move through an experimental system in a controlled way.