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Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
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JoVE Journal Neuroscience
Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

DOI:
10.3791/1884-v

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04:41 min

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July 14, 2010

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, , , , ,
1Taub Institute for Research on Alzheimer’s Disease and Aging Brain,Columbia University

Chapters

  • 00:04Title
  • 00:35Introduction
  • 01:03Preparation of Oligomeric Beta-amyloid 1-42
  • 03:03Hippocampal Slice Treatment & LTP Induction
  • 04:04Conclusion

Summary

Automatic Translation

Automatic Translation

One feature of Alzheimer's Disease is the elevation of Aβ1-42 peptide. Here we provide a protocol for preparing synthetic Aβ1-42 oligomers, which impairs hippocampal Long-Term Potentiation, a cellular correlate of memory. This procedure is useful for investigating mechanisms of Aβ-induced pathology and drug screening.

Tags

Oligomeric β-amyloid1-42Induction Of Synaptic Plasticity ImpairmentHippocampal SlicesAlzheimer’s DiseaseAβ PeptideLong-term PotentiationLearning And MemorySynaptic DysfunctionsDrug ScreeningOligomerized Synthetic Aβ1-42 PeptideLTP DisruptionExperimental ParadigmControl Experiment

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