Pneumatic Capillary Gun
Pneumatic Capillary Gun - Related Videos
05:44
Gene-gun Transfection of Hippocampal Neurons
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Cited by 2 •
2006
10:01
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
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Cited by 2 •
2016
This work presents a simple and visual method to detect multi-nucleotide polymorphisms on a pneumatic droplet manipulation platform. With the proposed method, the entire experiment, including droplet manipulation and detection of multi-nucleotide polymorphisms, can be performed near 23 °C without the aid of advanced instruments.
12:02
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Cited by 8 •
2014
This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.
17:42
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
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Cited by 90 •
2008
We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.
08:50
Capillary Electrophoresis (CE)
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution. Analytes are separated due to differences in electrophoretic mobility, which varies with charge, solvent viscosity, and size. Traditional electrophoresis in gels is limited in the amount of voltage...
FAQs
The compressed gas generates a controlled pressure pulse that accelerates particles through a narrow capillary. As the particles leave the capillary, their momentum carries them across cellular barriers and into biological material. This direct physical transfer allows the delivery event to be localized rather than relying on chemical or biological carriers.
Particle momentum provides the force needed to cross barriers surrounding cells or tissues. That mechanism is especially relevant for samples described as difficult to penetrate, where indirect delivery approaches may be less effective. Because the particles travel directly into the target material, the instrument supports localized manipulation within a selected biological region.
DNA coating converts the particles into carriers for genetic material during impact. After delivery into biological material, the introduced DNA can support transient gene expression or genetic transformation, depending on the experimental objective. This capability connects the instrument’s physical propulsion mechanism with downstream genetic outcomes in cell biology, plant science, and biotechnology.
The instrument uses a rapid, direct physical event rather than depending primarily on chemical components or biological carriers to move material across cellular barriers. Its distinguishing features are the compressed-gas pulse, capillary-guided acceleration, and particle momentum. This contrast makes it useful when researchers need localized delivery or access to samples that are difficult to penetrate.
A general workflow consists of selecting microscopic particles, coating them with DNA when genetic delivery is intended, positioning them within the narrow capillary, and applying a controlled compressed-gas pulse toward the biological material. The impact then delivers the particles into cells or tissues, where researchers evaluate the intended physical or genetic effect.
Researchers may choose it for transient gene expression, genetic transformation, or localized manipulation of cells and tissues. The approach is relevant to cell biology, plant science, and experimental biotechnology, particularly when biological material presents penetration challenges. Its rapid operation and direct delivery mechanism provide an alternative pathway for studying or modifying selected biological samples.