Porcine Aortic Endothelial Cell Isolation
Porcine Aortic Endothelial Cell Isolation - Related Videos
04:50
Porcine Aortic Endothelial Cell (pAEC) Isolation: An Enzyme-based Method to Isolate Endothelial Cells from Harvested Porcine Abdominal Aorta
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In this video, we demonstrate the isolation of endothelial cells from the abdominal aorta of a pig model using one-step collagenase digestion. The endothelial cells can be cultured for further downstream applications.
08:20
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
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Cited by 75 •
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The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.
11:04
Isolation of Valvular Endothelial Cells
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Cited by 82 •
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We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
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Laser-assisted Selective Ablation of Porcine Corneal Endothelial Cells: A Technique to Induce Focused Porcine Corneal Endothelial Injury for Ex Vivo Studies
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This video demonstrates a non-invasive laser-assisted procedure to selectively damage the corneal endothelial cells in the porcine eye. The developed ex vivo model of endothelial cell damage can be used to study diseases of the corneal endothelium.
08:05
Isolation of Embryonic Ventricular Endothelial Cells
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Cited by 4 •
2013
Primary cell culture is a useful technique for analyzing specific populations of cells, particularly from transgenic mouse embryos at specific developmental stages. Herein, embryonic ventricles are dissected and dissociated, and antibody-conjugated beads recognize and separate out the endothelial cells for further analysis.
FAQs
The luminal surface is the site where endothelial cells are located and attached. Opening the vessel to expose this surface allows the release step to target the relevant cell layer rather than unrelated vascular tissue. Careful handling therefore supports recovery of cells suitable for studying endothelial phenotype, barrier behavior, inflammatory responses, and blood-contacting interactions.
Both approaches are used to detach adherent endothelial cells from the exposed luminal surface so they can be collected for culture. Their shared purpose is to separate the target cell population from the vessel lining while preserving material that can attach and grow under supportive culture conditions. The resulting preparation enables downstream vascular studies.
Porcine vessels share important structural and physiological features with human arteries, giving cells isolated from pig aortas relevance to human vascular research. This relationship supports studies that connect basic endothelial biology with vascular injury, cardiovascular disease mechanisms, tissue engineering, and the development of materials intended to contact blood.
Cultured cells can provide information about endothelial phenotype, barrier behavior, and inflammatory responses. They can also be evaluated in relation to blood-contacting materials, allowing investigators to examine how endothelial cells interact with engineered surfaces or devices. These readouts connect the isolated culture to both cellular function and vascular material performance.
The workflow generally begins with sterile removal of the aorta, followed by opening the vessel and exposing its luminal surface. Adherent cells are then released through enzymatic or mechanical means and collected. Finally, the preparation is placed under culture conditions that support endothelial attachment and growth, producing cells for subsequent experiments.
Sterile handling is part of the preparation because the collected cells must remain suitable for laboratory culture and analysis. After release, culture conditions need to support cell attachment and growth so the recovered population can be expanded or maintained for experiments. These steps determine whether the isolation yields a usable endothelial model.
Researchers can apply the preparation to vascular biology, endothelial function, and vascular injury studies. It also supports tissue engineering and translational biomaterials development, particularly when investigators need to examine endothelial interactions with blood-contacting materials. Because the model comes from porcine arteries, it helps connect vessel-based biology with cardiovascular research questions relevant to human arteries.