Reporter Gene Transcription
Reporter Gene Transcription - Related Videos
04:25
Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene
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2025
This video demonstrates an in vivo assay in bacteria to study the interaction of RNA-binding proteins (RBPs) with RNA. The bacterial cells are transformed with two plasmid constructs — a binding-site plasmid encoding an mRNA containing a fluorescent reporter gene downstream of an RBP-binding site — while an RBP plasmid expresses the RBP proteins under the control of an inducer. Upon inducer-mediated increase in RBP production, the RBPs bind to the mRNA, resulting in translational repression of...
03:50
A Lentiviral Transcriptional Reporter System to Generate a Glioblastoma Stem Cell Differentiation Reporter Line
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2025
This video demonstrates a technique to generate a glioblastoma stem cell reporter line to identify the differentiation of glioblastoma stem cells (GSCs) into astroglia. It outlines the steps involved in seeding GSCs, transducing them with a lentivirus encoding GFP, and determining GFP reporter expression through flow cytometry.
03:08
Production of Reporter-Containing Ebola Virus Transcription- and Replication-Competent Virus-Like Particles
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2026
Source: Hoenen, T., et al. Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes. J. Vis. Exp. (2014)This video demonstrates how a tetracistronic minigenome plasmid mimicking the Ebola virus genome is transfected into human cells along with helper plasmids. Inside the cells, viral proteins and luciferase are expressed, leading to the assembly and budding of non-pathogenic, replication-competent virus-like...
09:22
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Cited by 11 •
2013
This protocol describes an experimental procedure for the rapid construction of artificial transcription factors (ATFs) with cognate GFP reporters and quantification of the ATFs ability to stimulate GFP expression via flow cytometry.
02:11
Reporter Genes
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2021
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell. Commonly used reporter...
FAQs
Control begins when the selected promoter or other regulatory sequence responds to cellular signals or an experimental condition. That response changes transcription of the engineered reporter gene, altering the amount of messenger RNA produced. Translation then yields a reporter protein whose fluorescence, luminescence, or other measurable output reflects the upstream regulatory response.
A time series shows how reporter activity changes as cells experience a signal, treatment, or other condition. Comparing signal intensity at multiple time points can reveal when regulation begins, whether activity increases or decreases, and how long the response persists. This makes the approach useful for examining dynamic changes in gene regulation.
If a reporter is controlled by a regulatory sequence responsive to a transcription factor, changes in its measurable output can indicate altered transcription-factor activity. Researchers can therefore examine how that factor influences promoter activity under different cellular signals or treatments. The resulting measurements also help connect transcription-factor function with broader signaling pathways and regulatory mechanisms.
Researchers place the reporter gene under a selected promoter or regulatory sequence, then examine the engineered system in living cells or another biological sample. They expose the system to relevant cellular signals or experimental conditions and measure the resulting fluorescence, luminescence, or other output. Signal intensity is then compared across conditions or time points.
Reporter assays can compare conditions that are expected to alter gene regulation, including different cellular signals or treatments described in the experiment. Because the reporter output is measurable, researchers can evaluate whether promoter activity changes between conditions and follow those differences over time. This provides a way to assess regulatory responses without relying on a single observation.
In biology, this approach supports investigations of gene expression, promoter activity, transcription-factor function, signaling pathways, and cellular responses to treatments. Its usefulness comes from connecting an engineered regulatory sequence to a detectable output in living cells or biological samples. Measurements can therefore help characterize regulatory mechanisms and track how cellular processes respond to experimental conditions.