Reporter Proteins
Reporter Proteins - Related Videos
03:15
Screening of Cyclic di-GMP Modulators Using Bacteria Expressing a Fluorescent Reporter Protein
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2025
Source: Rugjee, K. N., et al. Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa. J. Vis. Exp. (2016).This video demonstrates the screening of cyclic di-GMP modulators using genetically modified bacteria that express green fluorescent protein (GFP) under the control of a c-di-GMP-sensitive promoter. Fluorescence is measured in a multi-well plate containing different test compounds. Compounds that inhibit c-di-GMP...
04:11
A FRET Flow Cytometry Technique to Detect Tau-Seed Induced Reporter Protein Aggregation
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2025
The video demonstrates a fluorescence resonance energy transfer (FRET) flow cytometry assay to detect the seeding activity of protein aggregates isolated from biological samples. Mammalian cells expressing tau reporter proteins are incubated with liposome transduction complexes containing tau seeds. These seeds mediate the aggregation of the reporter proteins, leading to generating a FRET positive signal in the flow cytometer.
08:07
Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
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2024
Here, we describe a newly developed multiplex fluorescent immunoassay that uses a dual-reporter flow cytometric system to concurrently detect two unique spike protein epitopes on intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles that had been captured by angiotensin-converting enzyme-2-coupled magnetic microspheres.
03:50
A Lentiviral Transcriptional Reporter System to Generate a Glioblastoma Stem Cell Differentiation Reporter Line
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2025
This video demonstrates a technique to generate a glioblastoma stem cell reporter line to identify the differentiation of glioblastoma stem cells (GSCs) into astroglia. It outlines the steps involved in seeding GSCs, transducing them with a lentivirus encoding GFP, and determining GFP reporter expression through flow cytometry.
08:46
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Cited by 34 •
2015
A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.
FAQs
Reporter Proteins can be configured in two principal ways: a reporter gene may be placed under a regulatory sequence of interest, or the reporter may be fused to a target protein. The first arrangement links signal to gene or pathway activity, whereas the second helps track the target protein’s location. This distinction guides experimental design.
The three signal types provide different readout formats rather than a single measurement mode. Fluorescent reporters can be examined through microscopy, bioluminescent reporters through suitable signal detection, and enzymatic reporters through other assays. Together, these options let investigators measure activity in living systems using imaging, spectroscopy, or assay-based approaches.
Signal intensity can be interpreted as an indication of how strongly the associated gene or cellular pathway is active, provided the reporter is linked to the relevant regulatory sequence or target protein. This makes the readout useful for comparing biological activity across conditions or locations, while preserving the distinction between expression control and protein localization.
To build a reporter experiment, researchers first connect the reporter gene with the regulatory sequence being investigated or fuse the reporter to the target protein. They then observe the resulting fluorescence, bioluminescence, or enzymatic signal with microscopy, spectroscopy, or another assay. This workflow converts gene, pathway, or localization questions into measurable outputs.
During development, activity can change across cells, locations, or stages. A reporter linked to a relevant regulatory sequence can make those otherwise invisible changes observable, while signal strength can provide an indication of activity level. This enables researchers to examine when and where biological programs operate in living systems rather than relying only on an inferred molecular event.
Reporter systems can be incorporated into studies of disease mechanisms, genetic constructs, and potential therapeutics. Their measurable signals help investigators evaluate whether a construct or pathway is active and follow biological activity in living systems. Because outputs can report expression, localization, or pathway activity, the same general strategy can connect molecular changes with experimental outcomes.