Retinal Ganglion Cells
Retinal Ganglion Cells - Related Videos
05:26
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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Cited by 5 •
2011
We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.
13:02
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
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Cited by 26 •
2011
This article provides a description of how to dissect and record from the isolated retinal preparation in mouse. In particular, we describe how to record light responses from a fluorescently labeled ganglion cell population and subsequently identify and analyze its morphology.
11:26
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
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Cited by 8 •
2017
Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.
03:20
Identification of Mouse Retinal Ganglion Cell Subtypes Through Fluorescent Tracing
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2025
Source: Laboissonniere, et. al. Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells. J. Vis. Exp. (2017). This video demonstrates a fluorescent tracing technique to identify retinal ganglion cell (RGC) subtypes in a transgenic mouse retina. The process involves securing the isolated retina in a recording chamber, maintaining cell viability with oxygenated perfusion, and targeting fluorescently labeled RGCs using a pipette with a tracer. By analyzing dendritic morphology and...
03:10
Generation of Primary Retinal Ganglion Cell Cultures From Zebrafish Embryos
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2025
The video demonstrates a method for culturing primary retinal ganglion cells from zebrafish embryos. Early-stage embryos undergo dissection to isolate the eyes, which are subsequently mechanically dissociated into a suspension of retinal ganglion cells. This cell suspension is cultured on coverslips coated with adhesive proteins to facilitate axon development.
FAQs
Receptive-field integration allows a retinal ganglion cell to combine signals arriving from connected retinal circuits rather than responding to an isolated input. This processing helps represent differences in contrast, changes associated with motion, and variations in light intensity. Consequently, the cell’s output carries organized information about visual features that can be analyzed by the brain.
Bipolar and amacrine cells provide the upstream signals that retinal ganglion cells combine before producing an output. Their input contributes to the integration of activity across a receptive field, allowing the ganglion cell to reflect multiple aspects of the visual scene. This arrangement makes RGCs important points for studying how retinal circuits transform visual information.
Action potentials provide the electrical signals that retinal ganglion cells send along their axons through the optic nerve. This converts locally integrated retinal activity into messages that can travel from the eye toward the brain. Examining this output helps researchers connect cellular signaling in the retina with the transmission of visual information beyond the eye.
RGCs provide a cellular entry point for investigating how retinal circuits encode contrast, motion, and light intensity. Researchers can examine how signals integrated within receptive fields correspond to these features and how the resulting action-potential output represents them. This makes the cells useful for linking neural activity with specific components of visual processing.
Retinal ganglion cell dysfunction or death contributes to glaucoma, making these neurons central to studies of disease-related vision loss. Investigations can focus on how impaired RGCs affect the transmission of visual messages and on strategies intended to protect them. This research context connects retinal cell biology with neuroprotection and efforts to preserve sight.
Research on retinal ganglion cells supports several goals beyond describing normal visual processing, including neuroprotection, retinal repair, and strategies for restoring sight. Their role in carrying processed retinal signals provides a basis for evaluating how damage changes visual communication. Studying both their signaling and vulnerability therefore links basic biology with approaches to retinal recovery.