Scanning Mobility Particle Sizer
Scanning Mobility Particle Sizer - Related Videos
11:18
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)
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Cited by 9 •
2017
In this work a practical guide is provided, describing the different steps to establish the coupling of SMPS and ICPMS systems, and how to use them. Three descriptive examples are presented.
07:47
Experimental Protocol to Investigate Particle Aerosolization of a Product Under Abrasion and Under Environmental Weathering
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2016
In this article, an experimental protocol to investigate particle aerosolization of a product under abrasion and under environmental weathering is presented. Results on the emission of engineered nanomaterials, in the form of aerosols are presented. The specific experimental set-up is described in detail.
11:36
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
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Cited by 17 •
2012
We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.
02:28
Leaky Scanning
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2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
06:57
Electrophoretic Mobility Shift Assay (EMSA)
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2023
The electrophoretic mobility shift assay (EMSA) is a biochemical procedure used to elucidate binding between proteins and nucleic acids. In this assay a radiolabeled nucleic acid and test protein are mixed. Binding is determined via gel electrophoresis which separates components based on mass, charge, and conformation. This video shows the concepts of EMSA and a general procedure, including gel and protein preparation, binding, electrophoresis, and detection. Applications covered in this video...
FAQs
Charging gives particles an electrical property that allows the differential mobility analyzer to separate them according to electrical mobility. This separation supports size-resolved measurement rather than treating the aerosol as one undifferentiated population. In biological studies, the approach helps distinguish particle-size ranges within bioaerosols, aerosolized cells, biomolecules, or materials produced during laboratory and environmental processes.
The differential mobility analyzer selects particles according to electrical mobility as the instrument scans across particle sizes. The condensation particle counter then detects and counts the classified particles. Combining classification with counting produces information about both the relative particle-size distribution and particle concentration, allowing investigators to compare distinct aerosol populations or follow how those populations change.
A changing distribution shows that the aerosol population is shifting across measured size ranges or that particle concentrations are changing over time. Such patterns can help characterize particles generated by biological or environmental processes and assess whether a process produces a stable or changing aerosol. The result is a time-resolved view of population changes rather than a single concentration value.
The measurement sequence begins by charging particles in the aerosol, followed by electrical-mobility separation in the differential mobility analyzer. The instrument scans across particle sizes, while the condensation particle counter detects and counts particles selected during the scan. The resulting measurements are used to construct particle-size distributions and concentrations for the aerosol under study.
Biologists may use this system when they need to characterize bioaerosols, aerosolized cells, or aerosolized biomolecules. It is also relevant for particles generated during laboratory or environmental processes. These measurements provide particle-size and concentration information that can support studies of airborne biological materials and environmental exposures, especially when particle populations may vary during the process.
Size-resolved concentration data can clarify which particle ranges are present in an aerosol and how those ranges change. That information supports evaluation of particle transport, identification of inhalation-relevant size ranges, and assessment of filtration performance. In biological and environmental work, comparing distributions before and after a process can reveal changes in the measured particle population.