Skin Ulcer Models
Skin Ulcer Models - Related Videos
11:36
Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs
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Cited by 40 •
2012
Optimization of the experimental hamster model for cutaneous leishmaniasis by intradermal injection of Leishmania promastigotes at the dorsal skin. This approach is useful during inoculation, follow-up, characterization of lesions, application of treatments and obtaining of clinical samples. Locomotion, search for food and water, play and social activities are preserved.
03:02
Chemical-induced Two-stage Skin Carcinogenesis Model: An Experimental In Vivo Mouse Model of Skin Cancer
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2023
This video describes the procedure to induce two-stage skin carcinogenesis to study tumor initiation by topical application of chemicals. In the first step, a mutagen, DMBA, is applied onto the skin of a mouse model which initiates tumor formation by causing mutations in the stem cells, followed by the application of a growth stimulator, TPA, which accelerates skin papilloma formation.
04:07
Generation of Organotypic Full Skin Reconstructs: A Procedure to Establish 3D Skin Model Using Human Skin Samples
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2023
This video describes the detailed protocol for generating in vitro 3D organotypic skin reconstructs to study melanoma progression. This skin model mimics both the architecture and multicellular complexity of organs in vivo.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
11:02
The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression
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Cited by 111 •
2011
In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.
FAQs
The key mechanistic feature is that these models allow researchers to examine where normal wound repair becomes persistent or ineffective. Injury can disturb the usual progression from hemostasis and inflammation to tissue formation and remodeling, while reduced blood flow, infection, or metabolic dysfunction can further impair repair. This connects specific conditions with delayed healing responses.
Keratinocytes, fibroblasts, immune cells, and blood vessels contribute different aspects of tissue repair, so their interactions provide a multicellular view of healing. Keratinocytes relate to re-epithelialization, fibroblasts to connective-tissue repair and collagen deposition, immune cells to inflammatory responses, and blood vessels to vascularization. Studying these relationships helps explain why repair succeeds or remains impaired.
The choice of model determines which disruptive condition researchers can examine. Cultured skin cells and engineered tissues support controlled study of cellular or tissue-level responses, whereas animal models can reproduce injury together with factors such as reduced blood flow, infection, or metabolic dysfunction. Comparing these systems helps match the experimental platform to the biological question and desired outcome.
At a broad level, researchers select a cultured-cell, engineered-tissue, or animal platform, introduce or examine a condition that disrupts repair, and then follow biological changes during healing. The study can focus on inflammatory responses, tissue formation, remodeling, or interactions among relevant cell types. Researchers subsequently assess measurable features such as closure, re-epithelialization, collagen deposition, or vascularization.
Useful outcomes include wound closure, re-epithelialization, collagen deposition, and vascularization. Together, these measurements represent different aspects of repair: restoration of the surface, rebuilding of connective tissue, and development of blood-vessel involvement. Examining several outcomes rather than relying on closure alone provides a broader assessment of how a treatment or biological condition affects healing.
These models are used when researchers need to examine how therapeutic strategies affect persistent wound repair. Biomaterials, drugs, and cell-based treatments can be evaluated against outcomes such as wound closure, re-epithelialization, collagen deposition, and vascularization. Because the systems can incorporate cellular, tissue, or animal contexts, they also help investigate treatment effects within the biological processes underlying impaired healing.