Slide Scanning Microscope
Slide Scanning Microscope - Related Videos
03:25
Imaging of Neuronal Mitochondria Using a Serial Block-Face Scanning Electron Microscope
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2025
Source: Mukherjee, K., et. al. Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy. J. Vis. Exp. (2016)This video demonstrates the preparation, imaging, and analysis of mouse brain tissue using a serial block-face scanning electron microscope (SBFSEM) to visualize mitochondrial ultrastructure, enabling precise mapping of their morphology and spatial distribution in axonal and dendritic neuronal compartments.
11:14
Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope
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Cited by 4 •
2016
The optical, electrical, and structural properties of dislocations and of grain boundaries in semiconductor materials can be determined by experiments performed in a scanning electron microscope. Electron microscopy has been used to investigate cathodoluminescence, electron beam induced current, and diffraction of backscattered electrons.
09:33
Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System
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Cited by 2 •
2018
Here we describe a protocol for the automated segmentation of fluorescently labeled tissues on slides using a widefield high-content analysis system (WHCAS). This protocol has wide-ranging applications in any field which involves the quantitation of fluorescent markers in biological tissues including the biological sciences, medical engineering, and health sciences.
11:00
Hand Controlled Manipulation of Single Molecules via a Scanning Probe Microscope with a 3D Virtual Reality Interface
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Cited by 6 •
2016
We demonstrate the precise manipulation of individual organic molecules on a metal surface with the tip of a scanning probe microscope driven in 3D by the experimenter's hand using a motion capture system and fully immersive virtual reality goggles.
12:54
Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
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Cited by 13 •
2016
Coherent anti-Stokes Raman scattering (CARS) microscopy based on inherent vibration of molecule bonds permits label-free chemically selective live cell imaging. This work presents the implementation of a complementary microscopy technique on a standard multiphoton laser scanning microscope based on a femtosecond Ti:sapphire laser and an OPO laser.
FAQs
The system moves across the slide in a controlled sequence and captures neighboring fields through the microscope objective and camera. These individual images are then combined into a continuous whole-slide image, allowing users to examine specimen regions together rather than as isolated views. This arrangement supports consistent inspection of tissue organization and cell morphology across a large biological specimen.
The motorized stage positions different areas of the slide beneath the optical system, while the microscope objective provides the selected level of magnification and the camera records each field. Coordinating these components lets the instrument sample the specimen systematically. Selectable magnification also allows users to examine either broader tissue organization or finer cellular features within the same digital slide.
Different biological questions require different visual scales. A broader view can reveal tissue organization, whereas a higher-magnification view can support assessment of cell morphology. Selectable magnification lets investigators shift between these levels while working with the same scanned specimen, making the resulting digital image useful for both general structural examination and more focused biological analysis.
Once the specimen has been converted into a digital whole-slide image, it can be stored and incorporated into computational image-analysis workflows. This supports quantitative examination rather than relying only on visual inspection. In biology, such analysis can contribute to studies of tissue organization and cell morphology, while digital storage also preserves a standardized record for later review or comparison.
A prepared microscope slide is positioned on the instrument’s motorized stage, and the system scans across the specimen while the objective and camera capture sequential fields. The fields are combined into a whole-slide image, often at a selected magnification. The resulting file can then be viewed, analyzed, stored, or shared as a digital representation of the specimen.
The method is particularly useful for histology, tissue organization studies, and cell morphology assessment. Histology researchers can examine digitized specimen structure, while studies of tissue organization can compare patterns across larger regions. Cell-focused investigations can use selectable magnification and digital viewing to inspect morphological features and support quantitative image analysis.
Digital slides provide a standardized record that can be stored and revisited without repeatedly relying on the physical slide for viewing. They can also be shared rapidly among researchers, supporting collaborative examination and discussion. These capabilities are valuable when teams need consistent documentation, distributed review, or access to the same specimen image during teaching and research.
Whole-slide imaging makes it practical to organize digital records for large-scale specimen comparison. Researchers can review tissue organization and cell morphology across multiple specimens and apply quantitative image-analysis workflows to the stored images. This supports more systematic comparison than examining specimens only as separate microscope views, while retaining digital documentation for subsequent analysis and review.