Stromal Tissue Removal
Stromal Tissue Removal - Related Videos
05:48
Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
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Cited by 7 •
2014
Tissue engineered fibroblast-derived native matrix is an emerging tool to generate a stromal substrate which supports epithelial cell proliferation and differentiation. Here a protocol applying this methodology to assess the impact of different stromal cell types on tumor cell biology is presented.
12:40
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
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Cited by 91 •
2016
Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.
02:43
Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
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2023
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Cited by 151 •
2013
Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.
08:37
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
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Cited by 86 •
2017
This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.
FAQs
Collagenase breaks down extracellular-matrix components that help hold stromal tissue together. This loosens the supportive compartment so researchers can separate it from functional cells or structures more efficiently. The digestion must be controlled, because the purpose is not simply to disrupt tissue, but to reduce stromal material while limiting damage to the target cells being analyzed.
Mechanical dissociation physically separates tissue components, whereas enzymatic digestion uses collagenase to weaken extracellular-matrix connections. Researchers may combine both approaches because careful dissection can first remove or divide obvious stromal regions, while digestion helps release cells from remaining matrix. The selected balance influences how effectively stromal material is reduced and how well target cells are preserved.
Extracellular matrix contributes to the structural organization of stromal tissue and can obstruct access to the functional cells or structures within a sample. Reducing this matrix simplifies the biological material available for analysis. At the same time, excessive disruption may compromise the target compartment, so matrix breakdown must be matched to the intended downstream assessment.
The decision depends on the biological question and the compartment of interest. Removing stromal tissue can improve characterization of functional cells and clarify their behavior, while retaining or examining stromal components can help investigate microenvironmental interactions. This distinction matters in studies of development, repair, and disease, where both cell-intrinsic properties and tissue context may influence interpretation.
A typical workflow begins with careful dissection to separate visible supportive regions from the functional compartment. Researchers may then apply mechanical dissociation, enzymatic digestion, or both, using collagenase when extracellular matrix must be loosened. Throughout the process, conditions are managed to limit damage to target cells. The resulting preparation can then support focused cellular, structural, or tissue-level analysis.
This preparation can support primary cell isolation, histological assessment, and organoid preparation. It is also useful when researchers need to examine tissue composition or behavior with less complexity from surrounding supportive material. By making particular compartments easier to study, the approach can help connect cellular characteristics with broader processes such as tissue organization and function.
Separating stromal and functional compartments helps researchers examine how the microenvironment influences biological behavior. Analyses can compare target cells with reduced stromal complexity or investigate stromal and parenchymal or epithelial compartments more distinctly. This is relevant to development, repair, and disease research because supportive tissue may shape the behavior of neighboring functional cells and structures.