Sutter Puller
Sutter Puller - Related Videos
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
0 Views •
Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
04:24
Injection of dsRNA into Female A. aegypti Mosquitos
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Cited by 8 •
2007
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
05:09
Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
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Cited by 35 •
2012
This surgical technique illustrates the injection of gene therapy vectors and stem cells into the subretinal space of the mouse eye.
07:23
Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
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Cited by 6 •
2010
We demonstrate our approach to finding potential enhancer elements from developmentally regulated genes and evaluating their function through mosaic zebrafish transgenesis.
13:39
Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
0 Views •
Cited by 20 •
2009
Direct intranuclear injection of cDNA is an effective transfection technique for post-mitotic cells. This method provides high levels of heterologous protein expression from single or multiple cDNA constructs and enables protein function to be studied in a physiologically relevant environment with a variety of single cell assays.
FAQs
Heating brings the glass capillary to a softened state in which it can deform, while the calibrated pulling force separates the softened material and creates tapered ends. The relationship between these two actions determines how narrowly and precisely the glass is shaped. Controlling both is therefore essential when producing micropipettes for experiments that require consistent access to cells or tissues.
Programmable settings allow the pulling process to be controlled so that the resulting micropipette has a selected tip geometry and resistance. These characteristics influence how the pipette performs during biological measurements or manipulation. Reproducible settings help produce comparable pipettes across experiments, reducing variation that could otherwise complicate interpretation of membrane measurements, intracellular recordings, or microinjection results.
Consistent fabrication reduces differences among pipettes used in related experiments. Because tip shape and resistance are controlled during pulling, repeatable production supports more comparable measurements and manipulations from one preparation to the next. This is particularly valuable in studies of membrane properties, cell signaling, and neural function, where variation in the tool can affect experimental consistency.
A glass capillary is positioned in the instrument, heated until the glass softens, and then subjected to a calibrated pulling force. The force divides the softened capillary and produces narrow, tapered tips. Programmable settings are used to control the resulting geometry and resistance, after which the fabricated micropipettes can be selected for the intended biological experiment.
Fabricated micropipettes support several types of biological work, including patch-clamp electrophysiology, intracellular recording, and microinjection. They can also be used in related cell and tissue studies requiring controlled manipulation or measurement. The appropriate pipette characteristics depend on the intended experiment, since programmable control provides different combinations of tip geometry and resistance.
When used in suitable experimental systems, these micropipettes support investigations of membrane properties, cell signaling, and neural function. Patch-clamp electrophysiology and intracellular recording provide approaches for examining cellular electrical behavior, while microinjection enables controlled delivery into cells. Reliable pipette fabrication strengthens these applications by making the physical tool more consistent across measurements and manipulations.