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Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

JoVE Journal
Medicine
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JoVE Journal Medicine

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

DOI:

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05:09 min

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November 25, 2012

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Katherine J. Wert², Jessica M. Skeie⁴, Richard J. Davis, Stephen H. Tsang³, Vinit B. Mahajan⁴

¹Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology,Columbia University , ²Institute of Human Nutrition, College of Physicians & Surgeons,Columbia University , ³Omics Laboratory,University of Iowa , ⁴Department of Ophthalmology and Visual Sciences,University of Iowa

Chapters

00:00Title
00:09Injection Device Assembly
01:04Aliquot Vector or Cells
01:28Subretinal Injection
02:00Sclerotomy
02:54Subretinal Injection
04:51Summary

Summary

Automatic Translation

This surgical technique illustrates the injection of gene therapy vectors and stem cells into the subretinal space of the mouse eye.

Tags

Subretinal Injection Gene Therapy Vectors Stem Cells Perinatal Mouse Eye Blindness Retinal Degenerative Diseases Autologous Transplantation Age-related Macular Degeneration (AMD)Iris Pigment Epithelial Cell Transplantation RPE65 Gene Replacement Therapy Leber’s Congenital Amaurosis RPE Cell Transplantation Stargardt’s Disease Animal Models Therapeutic Efficacy

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