Target Enrichment
Target Enrichment - Related Videos
10:44
Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
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Cited by 1 •
2025
Here, we present a protocol to enable whole-genome sequencing of bacterial sexually transmitted infections from clinical samples using target enrichment. This novel, syndromic panel-based method overcomes challenges of abundant human DNA and low bacterial loads, facilitating genomic surveillance for Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, and Mycoplasma genitalium.
03:08
Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
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2025
The current work describes a protocol for running the Pathway2Targets algorithm, an R script that predicts and prioritizes therapeutic targets based on the profile of intracellular signaling pathways generated by comparing case versus control samples from a bulk RNA-sequencing experiment.
01:34
Environmental Enrichment for Rodents
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Environmental enrichment involves objects and practices that encourage natural behaviors, improve psychological well-being, and promote better welfare in laboratory rodents. Environmental enrichment should be tailored to the...
01:27
Environmental Enrichment in Rabbits and Pigs
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Importance of Environmental Enrichment Environmental enrichment is a fundamental component of laboratory animal care and welfare. It provides animals with opportunities to express species-specific behaviors, reduces stress, and...
10:12
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
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Cited by 1 •
2018
This protocol is to recover and prepare rare target cells from a mixture with non-target background cells for molecular genetic characterization at the single-cell level. DNA quality is equal to non-treated single cells and allows for single-cell application (both screening based and targeted analysis).
FAQs
In probe-based Target Enrichment, complementary probes recognize matching DNA or RNA sequences within a complex sample. Hybridization creates a sequence-selective association, after which target-associated material can be captured and separated from unwanted molecules. This selectivity increases the fraction of informative sequences available for downstream sequencing, focusing analysis on predefined genomic regions.
Probe capture and primer-based amplification enrich targets through different molecular routes. Hybridization uses complementary probes to associate selected sequences with capture material, whereas sequence-specific primers direct amplification of the regions they match. Both approaches increase target representation, but they do so through selection and separation in the first case and targeted copying in the second.
Selectivity matters because a complex sample contains many molecules outside the regions under investigation. Removing unwanted material through probe capture or emphasizing matched sequences through primer-based amplification increases the relative representation of informative molecules. The resulting sequencing effort is concentrated on chosen regions instead of being distributed across unrelated genomic content.
A basic probe-capture workflow brings complementary probes into contact with DNA or RNA from a complex sample, allowing target sequences to hybridize. The associated target material is then captured and separated from unwanted molecules. The enriched fraction can subsequently undergo sequencing, so the resulting data emphasize genomic regions selected at the beginning of the workflow.
Target enrichment is useful when the biological question concerns a defined set of sequences rather than the entire genome. Supported applications include variant detection, gene panel analysis, and pathogen identification. It can also focus experiments on genomic regions with clinical or biological importance, directing sequencing resources toward information most relevant to the study.
For a study centered on defined genomic regions, Target Enrichment directs sequencing toward selected sequences rather than surveying the entire genome. This focus can improve coverage while reducing sequencing cost and data-processing demands. Whole-genome analysis is broader in scope, whereas enrichment prioritizes detailed analysis of specified regions considered informative for the investigation.