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PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
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Bioengineering
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JoVE Journal Bioengineering
PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

DOI:
10.3791/55869-v

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10:58 min

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July 27, 2017

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, , ,
1Department of Biochemistry and Molecular Biology,The Pennsylvania State University, 2Department of Chemistry,The Pennsylvania State University

Chapters

  • 00:05Title
  • 01:04Fabricating Micropatterned PDMS Blocks
  • 02:14Preparing Small Unilamellar Vesicles (SUVs)
  • 03:29Assembling the Microfluidic Device
  • 04:42Forming Supported Lipid Bilayers (SLBs)
  • 07:56Testing PLC-δ1 PH Domain Interction with PI(4,5)P2-containing SLBs
  • 08:49Results: Assessing PLC-δ1 PH Domain: Phosphoinositide Interaction with PIP-on-a-chip Assay
  • 09:56Conclusion

Summary

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Here we present a supported lipid bilayer in the context of a microfluidic platform to study protein-phosphoinositide interactions using a label-free method based on pH modulation.

Tags

Protein-phosphoinositide InteractionsLabel-freeMembrane InteractionsPIP-on-a-chip AssayPDMSPhosphatidylcholinePhosphatidylinositol 45-bisphosphatePH-sensitive Fluorescent ProbeLipid VesiclesLabel-free MonitoringBiologically RelevantTherapeutic TargetsMembrane Proteins

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