Telogen Follicle Selection
Telogen Follicle Selection - Related Videos
06:51
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Cited by 23 •
2016
An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
10:31
Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
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Cited by 2 •
2025
This protocol describes a novel three-dimensional (3-D) culture model using a tyramine-linked hyaluronan hydrogel to encapsulate and culture preantral follicles from the mouse ovary. We also detail two approaches to ovarian follicle cryopreservation by vitrification.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
10:48
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
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Cited by 9 •
2017
A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.
08:44
The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation
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Cited by 12 •
2013
Stage-specific isolation of mid-to-late Drosophila follicles is useful for a variety of purposes. Such follicles develop in culture, which allows for genetic and/or pharmacologic manipulations to be coupled with in vitro development assays and live imaging. Additionally, follicles can be used for molecular studies, such as isolating mRNA and protein.
FAQs
The dermal papilla and surrounding niche provide activation signals that can move epithelial cells from relative quiescence toward a new anagen phase. Examining selected follicles allows investigators to ask whether these local signals are associated with cycle re-entry. This is useful for connecting tissue-level communication with the start of renewed follicle growth.
The retained club hair provides a characteristic feature of the resting follicle and helps distinguish the state being examined from later growth activity. Its presence should be considered alongside the follicle’s largely quiescent epithelial cells, because interpretation depends on recognizing both retention and low epithelial activity. This supports focused analysis of telogen biology.
It can help researchers examine how a follicle changes from a relatively inactive epithelial state to renewed growth. In particular, the approach supports analysis of stem-cell activation, follicle regeneration, and signals arising from the dermal papilla or surrounding niche. These observations clarify how tissue-level events contribute to hair-cycle transitions.
Researchers identify follicles displaying telogen-associated features, isolate them, and then use the selected material for focused biological analysis. The resulting samples can be examined in relation to epithelial quiescence, retained club hair, activation signals, or later entry into anagen. This focused workflow helps connect follicle state with specific experimental questions.
The approach is suited to studies of hair-cycle regulation, follicle regeneration, and the signals that control stem-cell activation. It also supports investigation of skin biology and mechanisms associated with hair loss. By concentrating on follicles before renewed growth begins, researchers can examine processes that precede visible follicle activity.
Selected telogen follicles provide a system for examining how follicle growth and cycling respond to biological conditions or experimental interventions. The same framework can contribute to wound-repair studies by analyzing follicle regeneration and tissue signals within skin. It also helps evaluate treatments intended to alter follicle growth or the timing of cycling.