Temperature Controlled Rearing
Temperature Controlled Rearing - Related Videos
06:43
Esophageal Heat Transfer for Patient Temperature Control and Targeted Temperature Management
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Cited by 6 •
2017
This study presents a novel method to provide efficient patient temperature control for cooling or warming patients. A single use, triple lumen device is placed into the esophagus, analogous to a standard orogastric tube, and connects to existing heat exchange units to perform automatic patient temperature management.
06:09
Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging
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2016
The social amoebae Dictyostelium discoideum has recently been established as a system to study protein misfolding and proteostasis. Here, we describe a new imaging-based methodology to study temperature-induced protein aggregation and the cellular stress response in D. discoideum.
01:23
Physical Methods for Controlling Microbial Growth: Temperature
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2025
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
13:03
Protocol for Mosquito Rearing (A. gambiae)
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Cited by 64 •
2007
This video illustrates the general techniques used to rear Anopheles gambiae in the laboratory. The methods for caring for laboratory mosquitoes are demonstrated through all stages of the organism's life cycle from larvae to pupae to blood-feeding adults.
10:11
Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
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Cited by 1 •
2021
This protocol details the use of a feedback temperature-controlled heating system to promote lipid monolayer assembly and droplet interface bilayer formation for lipids with elevated melting temperatures, and capacitance measurements to characterize temperature-driven changes in the membrane.
FAQs
Temperature acts on several biological processes at once, including metabolic activity, enzyme function, developmental rate, and survival. Consequently, changing or stabilizing the thermal condition can alter how organisms progress through life stages and how well they persist. Keeping temperature defined helps investigators attribute observed differences to biological responses rather than uncontrolled thermal variation.
Sensors provide ongoing information about the chamber or incubator environment, while a control system adjusts heating or cooling. This feedback limits departures from the selected condition instead of relying on a single initial setting. Reduced variation supports more consistent growth and makes results easier to compare among cultures or experimental groups.
A defined thermal condition gives researchers a common environment in which responses at different life stages can be examined. The same approach supports studies of thermal tolerance, meaning how organisms respond to temperature conditions, and adaptation. It therefore connects developmental observations with broader questions about survival and biological response.
Temperature should be treated as an experimental variable because it can affect metabolism, enzyme function, development, and survival simultaneously. If thermal conditions vary between groups or over time, those biological effects may be difficult to separate from other experimental differences. Defining and maintaining the condition makes comparisons more interpretable.
A basic workflow begins by placing the organism or culture in an incubator or environmental chamber, establishing the desired temperature, and using sensors to monitor it. The control system can then adjust heating or cooling when conditions shift. Maintaining that regulated setting supports consistent growth and creates a repeatable basis for measurements.
Researchers can use the method to examine biological responses under defined thermal conditions and compare those responses across life stages. Such comparisons can inform investigations of thermal tolerance and adaptation, while consistent rearing conditions help produce cultures suitable for laboratory studies in which repeatable observations are important.
Applications span developmental biology, ecology, genetics, microbiology, and organismal research. In each area, regulated thermal conditions can support a different objective, from following development to examining ecological or organismal responses. The shared value is that temperature remains a defined condition while researchers study growth, survival, adaptation, or culture consistency.