Termite Gut Microbes

Termite gut microbes are diverse bacteria, archaea, and single-celled eukaryotes that live symbiotically in the termite digestive tract and enable termites to use wood and other plant material as food. In the anaerobic hindgut, microbial enzymes break down cellulose and hemicellulose into simpler compounds, which are fermented into short-chain fatty acids that provide energy to the host; other microbes participate in hydrogen and methane metabolism. Studying these communities illuminates symbiosis, nutrient cycling, and the biological conversion of lignocellulosic biomass, with potential applications in biotechnology, biofuel research, and strategies for understanding termite ecology.

Termite Gut Microbes - Related Videos

Research

JoVE Journal - Biology

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes

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Cited by 18 •

2007

This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.

Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)

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Cited by 11 •

2010

We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).

Investigating the Microbial Community in the Termite Hindgut - Interview

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2007

Jared Leadbetter explains why the termite-gut microbial community is an excellent system for studying the complex interactions between microbes. The symbiotic relationship existing between the host insect and lignocellulose-degrading gut microbes is explained, as well as the industrial uses of these microbes for degrading plant biomass and generating biofuels.

Research

JoVE Journal - Biology
Free Sample

Layers of Symbiosis - Visualizing the Termite Hindgut Microbial Community

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Cited by 1 •

2007

Jared Leadbetter takes us for a nature walk through the diversity of life resident in the termite hindgut - a microenvironment containing 250 different species found nowhere else on Earth. Jared reveals that the symbiosis exhibited by this system is multi-layered and involves not only a relationship between the termite and its gut inhabitants, but also involves a complex web of symbiosis among the gut microbes themselves.

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

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Cited by 8 •

2018

Gut host-microbe interactions were assessed using a novel approach combining a synthetic oral community, in vitro gastrointestinal digestion, and a model of the small intestine epithelium. We present a method that can be adapted to evaluate cell invasion of pathogens and multi-species biofilms, or even to test probiotic formulations' survivability.

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