Tissue Culture Preparation
Tissue Culture Preparation - Related Videos
07:13
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
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Cited by 3 •
2016
The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue.
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
10:06
Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
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Cited by 32 •
2011
The procedure of preparing slices containing the adult mouse hypothalamic suprachiasmatic nucleus (SCN), and a rapid way to culture the SCN tissue in organotypic culture condition, are reported. Further, the measurement of oscillatory clock gene protein expression using dynamic luciferase reporter technology is described.
FAQs
Aseptic technique protects the culture during handling and transfer by limiting contamination. This matters because contamination can interfere with cell survival, tissue structure, or measured behavior. In practice, careful collection, trimming or dissociation, and movement into sterile vessels help maintain a usable preparation for downstream biological study.
They jointly create the controlled environment that supports cell survival and helps maintain tissue structure or function. The nutrient medium supplies suitable conditions for the preparation, while temperature and gas exchange provide additional controls during culture maintenance. Keeping these factors consistent improves the value of cultures for observing biological behavior.
The choice between trimming and dissociation should match the research aim. Retaining more of the original tissue can support observation of structure or function, while dissociation offers an alternative starting format when the planned culture requires it. This distinction helps investigators choose preparation strategies for primary cultures, explant models, or other biological studies.
A practical workflow begins with careful tissue collection, followed by trimming or dissociation as appropriate. The prepared material is then transferred into a sterile culture vessel containing suitable nutrient medium. Subsequent maintenance depends on controlled temperature and gas exchange, together with continued attention to contamination. This sequence connects physical preparation with conditions needed for survival and useful biological observations.
These cultures can support questions about cell behavior, development, disease, toxicity, or regenerative processes. The preparation serves as an experimental starting point rather than an endpoint: researchers maintain the material under controlled conditions and examine the resulting culture in relation to the biological question. This broad range explains its use across biology.
In tissue engineering research, preparation establishes a culture foundation from biological material that can be maintained under suitable conditions. The combination of sterile vessels, nutrient medium, controlled temperature, and gas exchange helps preserve tissue structure or function for examination. This makes the process relevant to regenerative studies and investigations involving engineered tissue systems.