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Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

DOI:
10.3791/2439-v

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10:06 min

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February 15, 2011

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, , ,
1Swedish Medical Nanoscience Center, Department of Neuroscience,Karolinska Institutet

Chapters

  • 00:05Title
  • 02:13Preparing for Slicing and Culturing
  • 03:17SCN Slicing Procedure
  • 06:06Organotypic SCN Culture
  • 08:09Results: Circadian Bioluminescence in SCN Slices
  • 09:27Conclusion

Summary

Automatic Translation

Automatic Translation

The procedure of preparing slices containing the adult mouse hypothalamic suprachiasmatic nucleus (SCN), and a rapid way to culture the SCN tissue in organotypic culture condition, are reported. Further, the measurement of oscillatory clock gene protein expression using dynamic luciferase reporter technology is described.

Tags

Slice PreparationLuciferase RecordingClock Gene ActivitySuprachiasmatic NucleusCircadian ClockPhysiologyBehaviorRetinaOptic NerveNegative Feedback LoopsClock GenesPeriod GenesCore ClockMembrane DepolarizationCalciumCAMPOscillationsGene ExpressionMetabolic ActivityElectrical ActivityIn Vitro StudyMolecular InvestigationsElectrophysiological InvestigationsMetabolic Investigations

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