Transcription Splicing
Transcription Splicing - Related Videos
11:19
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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Cited by 2 •
2016
Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.
02:18
Alternative RNA Splicing
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2020
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity. There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
01:32
RNA Splicing
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2019
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
07:18
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
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2019
A high throughput protocol for functional assessment of HIV efficient reactivation and clearance of latent proviruses is described and applied by testing the impact of interventions on HIV transcription and splicing. Representative results of the effect of latency reversing agents on LTR-driven transcription and splicing are provided.
09:58
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Cited by 25 •
2016
Here we describe a protocol aimed at investigating the impact of aberrant splicing on drug resistance in solid tumors and hematological malignancies. To this goal, we analyzed the transcriptomic profiles of parental and resistant in vitro models through RNA-seq and established a qRT-PCR based method to validate candidate genes.
FAQs
Conserved sequences at intron boundaries provide recognition points for the spliceosome, helping it distinguish regions that should be removed from neighboring exons that should remain. Accurate recognition is essential because the cutting and joining steps must occur at the correct positions. This boundary-selection mechanism directly influences the sequence of the mature messenger RNA.
The intron lariat is a temporary RNA structure formed when the spliceosome processes an intron. Its formation marks an intermediate stage between boundary recognition and exon joining, after which the neighboring exons are ligated. Examining this intermediate helps connect the molecular steps of RNA processing with the production of mature messenger RNA.
Alternative splicing changes which exons are combined in the processed RNA. Because different exon patterns can be selected, a single gene can produce multiple messenger RNA molecules and, consequently, multiple protein products. This flexibility gives cells additional ways to regulate gene expression and supports specialized functions in different cell types.
A focused analysis follows the sequence of processing events: recognition of conserved intron-boundary sequences, RNA cutting, formation of the intron lariat, and ligation of adjacent exons. Researchers can then compare the resulting exon combinations, including alternative patterns. This workflow links molecular events to the identity and potential diversity of mature messenger RNA products.
Abnormal RNA processing can change which exon combinations appear in messenger RNA and can therefore alter the protein products generated from a gene. Studying these changes helps investigators examine the molecular basis of diseases associated with abnormal splicing. The findings can also support the search for diagnostic markers and targeted therapeutic strategies.
Splicing contributes to gene regulation by determining how newly synthesized RNA is processed before it functions as messenger RNA. Alternative exon combinations allow related cells to produce different RNA and protein products from the same gene. This connection helps explain how molecular processing supports cellular specialization and why splicing patterns matter in biology research.