Wnt1-creer Mice
Wnt1-creer Mice - Related Videos
02:45
Assessment of Viral Fusion Inhibitors Using a Cre-Inducible Fluorescent Reporter
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2026
Source: Esposito, A. M.,et al. High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (2018)This video demonstrates a co-culture fluorescent assay to monitor cell-to-cell viral transmission. The assay uses a red-to-green reporter cassette to visualize viral membrane fusion and evaluate the efficacy of test compounds in blocking viral entry.
01:59
Cre-LoxP Mediated Induction of Cerebral Cavernous Malformations in a Mouse Model
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2025
In this video, a genetically modified mouse pup carrying the Ccm2 gene flanked by loxP sites and Cre recombinase fused to an estrogen receptor is used to induce cerebral cavernous malformations (CCMs). The administration of 4-hydroxytamoxifen activates Cre in endothelial cells, leading to the deletion of Ccm2 and the formation of CCMs in the brain.
08:19
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
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Cited by 15 •
2011
Phenotypic variation for traits can result from mutations in cis-regulatory element (CRE) sequences that control gene expression patterns. Methods derived for use in Drosophila melanogaster can quantitatively compare the levels of spatial and temporal patterns of gene expression mediated by modified or naturally occurring CRE variants.
05:19
Segmental Adeno-Cre Infection: A Technique to Generate Isolated Colorectal Cancer using Genetically Engineered Mouse Models
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2023
This video describes the generation of a colorectal mouse model by segmental adeno-cre infection. The infected colon segment leads to tumorigenesis within this segment of the mucosa, resulting in adenomas ultimately progressing to invasive and metastatic carcinoma. This model is an attractive platform for studies of cancer biology and preclinical therapeutic trials.
07:48
Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
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Cited by 3 •
2015
Intestinal epithelial stem cells (ISCs) are intermingled with Paneth cells. These cells are differentiated progeny of the ISC, which support the ISCs and provide antibacterial protection. Here we demonstrate how we used transgenic conditional mouse models to establish that Paneth cells play a crucial role in maintaining the intestinal epithelia.
FAQs
Recombination occurs in cells in which the Wnt1 promoter is active, giving the model a defined spatial pattern during development. This pattern is especially useful for examining the dorsal neural tube and neural crest rather than manipulating genes throughout the entire organism. The resulting regional control helps researchers associate gene activity with formation of particular tissues.
The arrangement of loxP sites determines how Cre-mediated modification affects the target DNA. Cre can remove the intervening segment or rearrange it, supporting conditional gene knockout or activation. Because these changes occur in Wnt1-expressing cells, investigators can examine how altering a gene in a selected developmental population changes tissue formation and later biological outcomes.
The initial Wnt1-expressing population gives rise to descendants that can populate developing structures. Genetic changes introduced in the original cells may therefore be observed in their descendants, extending the analysis beyond the cells that first expressed Wnt1. This feature supports lineage tracing and helps connect an early developmental population with the cell fates and tissues it generates.
The biological meaning of a genetic change depends on when Wnt1 promoter activity occurs during development. A modification introduced in an early Wnt1-expressing population can influence subsequent tissue formation and descendant cell behavior. Consequently, observed phenotypes should be interpreted as outcomes of gene function within a defined developmental lineage, not simply as effects in mature tissues.
A study first connects the desired genetic change to loxP sequences, then uses Cre activity associated with the Wnt1-expressing population to produce the planned modification. The resulting experiment focuses analysis on the dorsal neural tube, neural crest, or their descendants. This design links a gene's function to specific developmental tissues while avoiding an organism-wide interpretation.
The model is particularly relevant to neural development, craniofacial formation, and peripheral nervous system development. It also supports studies of cell fate in neural crest-related populations and their descendants. These applications allow researchers to investigate how gene manipulation influences the formation of nervous, craniofacial, and other tissues associated with the targeted developmental lineages.
Conditional gene manipulation in Wnt1-associated populations can reveal how a gene contributes to tissue formation and how developmental disruption may produce disease-related consequences. By restricting the genetic change to relevant cells and descendants, researchers can relate altered neural, craniofacial, or peripheral nervous system development to mechanisms underlying disease rather than relying only on broad genetic effects.
Lineage tracing identifies descendants of cells that experienced Wnt1 promoter activity, allowing investigators to follow their contribution to developing tissues. This approach provides evidence about where a developmental population goes and which structures it helps form. Combined with conditional gene manipulation, it can also show how changing gene function alters the fate or contribution of those descendants.