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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

DOI:
10.3791/53429-v

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07:48 min

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November 21, 2015

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, , ,
1European Cancer Stem Cell Research Institute,Cardiff University, 2Institut Curie

Chapters

  • 00:05Title
  • 00:33Intestine Dissection
  • 01:32Formalin Fixation
  • 02:25Methacarn Fixation
  • 03:38Whole Mount LacZ Visualization
  • 06:01Results: Representative Intestinal Epithelial Cell Characterization After Gene Manipulation
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

Intestinal epithelial stem cells (ISCs) are intermingled with Paneth cells. These cells are differentiated progeny of the ISC, which support the ISCs and provide antibacterial protection. Here we demonstrate how we used transgenic conditional mouse models to establish that Paneth cells play a crucial role in maintaining the intestinal epithelia.

Tags

Keywords: Intestinal Stem CellsLgr5Bmi-1Paneth CellsCre-lox Mouse ModelsIntestinal RegenerationIntestinal EpitheliumIntestinal Niche

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