Xenopus Tadpoles

Xenopus tadpoles are the aquatic larval stages of clawed frogs in the genus Xenopus, widely used to study vertebrate development because their embryos develop externally and remain accessible for observation and manipulation. After fertilization, coordinated cell division, gastrulation, tissue specification, and organ formation transform the embryo into a swimming tadpole, while conserved signaling pathways pattern structures such as the body axis and nervous system. In biology research, Xenopus tadpoles support experiments on gene function, embryonic patterning, regeneration, disease mechanisms, and environmental toxicity, linking cellular processes to whole-organism outcomes.

Xenopus Tadpoles - Related Videos

Research

JoVE Journal - Biology
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Spinal Cord Transection In Xenopus laevis Tadpoles

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2021

Xenopus laevis tadpole spinal cord transection is a relevant injury method to study spinal cord injury and regeneration by making a transverse cut that completely severs the spinal cord at the thoracic level.

Research

JoVE Journal - Developmental Biology

Generating Retinal Injury Models in Xenopus Tadpoles

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2023

We have developed several protocols to induce retinal damage or retinal degeneration in Xenopus laevis tadpoles. These models offer the possibility of studying retinal regeneration mechanisms.

Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

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2018

Xenopus tadpoles offer a unique platform to investigate the function of the nervous system in vivo. We describe methodologies to evaluate the processing of olfactory information in living Xenopus larvae in normal rearing conditions or after injury.

Research

JoVE Journal - Neuroscience
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Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

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2016

This article presents a convenient and rapid method for visualizing different neuronal cell populations in the central nervous system of Xenopus embryos using immunofluorescent staining on sections.

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

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2017

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

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