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JoVE Journal
Developmental Biology

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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
 

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Article DOI: 10.3791/55652 06:06 min May 25th, 2017
May 25th, 2017

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Summary

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

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Tags

Visualization Quantitative Analysis Embryonic Angiogenesis Xenopus Tropicalis Cardiovascular Biology Angiogenesis Regulation Fluorescent Dye Injection Micro Loader Glass Micro Pipette DiI Acetylated LDL Solution Pressure Controlled Injection System MS-222 Modified Barth's Saline Sylgard Mold Sedation Dorsal Fin Stimulation Embryo Positioning Minutian Pins Heart Pulsation
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