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JoVE Journal
Developmental Biology

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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
 

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Article DOI: 10.3791/55652
May 25th, 2017

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Summary May 25th, 2017

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

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