Yolk Cytoplasmic Injection
Yolk Cytoplasmic Injection - Related Videos
03:58
Embryo Microinjection: A Technique to Deliver a Compound into the Zebrafish Yolk
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2023
This video describes a technique of Embryo microinjection to deliver compounds into Zebrafish yolk sac.
09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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Cited by 3 •
2017
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
01:16
Cytoplasm
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2026
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.Protein Folding and MisfoldingThe cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein core...
01:24
Cytoplasm
0 Views •
2023
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules. Protein Folding and Misfolding The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
07:42
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
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Cited by 15 •
2011
In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.
FAQs
After injection, the introduced material can become distributed through the yolk cytoplasm and may transfer toward embryonic cells as development continues. This creates a developmental delivery route rather than requiring immediate placement inside a particular embryonic cell. The approach helps investigators examine how nucleic acids, proteins, tracers, or test compounds behave during early development.
The yolk cytoplasm provides a route for introducing material while minimizing direct disruption of developing cellular structures. This feature is important when an experiment requires reproducible delivery but does not depend on placing the material directly inside a specific embryonic cell. Yolk targeting can therefore preserve developmental organization while allowing introduced substances to influence subsequent embryonic processes.
Yolk Cytoplasmic Injection places the material in the yolk rather than directly into developing cellular structures. Its effects can therefore depend on distribution through the yolk and subsequent transfer toward embryonic cells during development. This differs from a direct cellular approach, where delivery occurs at the target cell itself and may cause more immediate structural disruption.
The technique can deliver several defined material types, including nucleic acids, proteins, tracers, and test compounds. These payloads support different experimental goals: nucleic acids and proteins can be used to examine gene function or signaling, tracers can help follow distribution, and test compounds can support developmental toxicity studies. The chosen material determines the biological question addressed.
A basic workflow uses a defined solution, a fine glass needle, and an egg or early embryo. The needle delivers the selected material into the yolk cytoplasm, after which development proceeds while the introduced substance can distribute or move toward embryonic cells. This sequence provides controlled delivery without requiring direct injection into the developing cellular structures.
Yolk Cytoplasmic Injection is useful for investigating embryogenesis, gene function, cell signaling, and developmental toxicity. Researchers can select nucleic acids, proteins, tracers, or test compounds according to the process under study. Because delivery occurs through the yolk during early development, the method connects controlled material introduction with biological changes that emerge as the embryo develops.