Zebrafish Base Editing
Zebrafish Base Editing - Related Videos
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
02:23
RNA Editing
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2020
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
07:03
Large Scale Zebrafish-Based In vivo Small Molecule Screen
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Cited by 17 •
2010
Zebrafish has emerged as a powerful in vivo platform for phenotype-based drug screens and chemical genetic analysis. Here, we demonstrate a simple, practical method for large-scale screening of small molecules using zebrafish embryos.
07:31
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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Cited by 11 •
2023
This protocol describes how to perform efficient adenine base editing without PAM limitation to construct a precise zebrafish disease model using zSpRY-ABE8e.
FAQs
The attached DNA deaminase chemically converts one DNA base into another at the sequence selected by the guide RNA and Cas protein. Because this reaction targets a nucleotide rather than cutting both DNA strands, it supports focused single-nucleotide changes. This chemical step is therefore central to testing how a specific DNA alteration affects zebrafish biology.
The guide RNA provides sequence-level direction by matching the chosen zebrafish DNA region, while the catalytically modified Cas protein helps bring the editing complex to that site. Their coordinated targeting determines which gene region receives the deaminase activity. This arrangement lets researchers connect a deliberately selected nucleotide change with later developmental, physiological, or behavioral observations.
A key distinction is that base editing generally avoids creating a DNA double-strand break, a feature associated with conventional nuclease-based editing. This allows researchers to pursue a targeted nucleotide change while reducing some unintended damage linked to break-based approaches. The comparison matters when a study requires a precise variant for interpreting gene function in a vertebrate model.
Researchers first select the zebrafish DNA sequence and design a guide RNA to direct the editing complex there. They then introduce the guide RNA and base-editor components into early embryos, allowing targeted variants to arise during development. Subsequent analysis examines the resulting animals for effects on development, physiology, or behavior, depending on the biological question.
The resulting variants can be assessed through several biological levels, including development, physiology, and behavior. These observations provide phenotypic evidence about how the altered nucleotide influences gene function. Examining multiple outcome types is useful because a variant may affect visible development, internal physiological processes, behavioral traits, or a combination of these features.
This approach enables researchers to model disease-associated mutations and test hypotheses about what particular genes do. By linking a defined nucleotide change to an observable phenotype in zebrafish, investigators can examine the biological consequences of that variant in a vertebrate system. The method therefore connects DNA sequence changes with organism-level evidence relevant to disease and normal development.