Microinjection Pump
Microinjection Pump - Related Videos
10:06
Personalized Needles for Microinjections in the Rodent Brain
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Cited by 3 •
2018
We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.
02:58
Rodent Brain Microinjection Using a Quartz Microneedle
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2025
Source: Paolone, G., et al. Personalized Needles for Microinjections in the Rodent Brain. J. Vis. Exp. (2018).This video demonstrates a rodent brain microneedle injection using a quartz microneedle with holes. The microneedle is attached to a syringe and micropump to control the delivery rate. The needle with the injection solution is inserted into the brain with the delivery speed set at low. Once positioned, the delivery speed is increased. The needle is withdrawn after a brief pause.
08:12
Zebrafish Microinjection Techniques
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2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
01:30
ATP Driven Pumps III: V-type Pumps
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2023
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient. The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
01:34
ATP Driven Pumps II: P-type Pumps
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2023
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V. A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
FAQs
A motor-driven syringe or plunger advances at a defined rate, producing controlled pressure and a steady liquid flow. Researchers can adjust the delivered volume, movement speed, and injection time to match the experimental target. These settings make injections more reproducible and help control how much material enters an individual cell, tissue, organoid, or model organism.
Selected volume, delivery speed, and injection duration directly affect the amount and timing of material introduced. The fine needle or micropipette also provides the delivery pathway, while the pressure generated by plunger movement regulates flow. Controlling these variables is important because inconsistent settings can increase experimental variability or affect the condition of injected samples.
Controlled pressure helps the operator deliver material steadily rather than releasing an unpredictable amount. This supports reproducible manipulation while minimizing sample damage, which is especially important when experiments compare gene function, tumor development, or drug response. Consistent pressure and flow also make differences between experimental groups more likely to reflect the treatment rather than injection variability.
The needle or micropipette forms the fine route through which liquid reaches the selected cell or tissue. Its use allows the pump to apply controlled delivery to individual biological targets instead of introducing material broadly. In cancer research, this supports targeted manipulation of cultured cells, organoids, and model organisms while helping limit unnecessary disturbance to surrounding samples.
A typical workflow includes loading the selected liquid into a motor-driven syringe or micropipette, choosing the intended volume, delivery speed, and injection time, and positioning the fine delivery instrument at the target. The operator then injects the material under controlled pressure and records the resulting manipulation. These settings can be standardized across samples to improve reproducibility.
The system can deliver drugs, nucleic acids, proteins, or tumor cells, depending on the experimental question. Researchers may introduce these materials into cultured cells, organoids, or model organisms to examine biological effects in a controlled setting. This flexibility supports studies of gene function, tumor development, treatment mechanisms, and responses to candidate therapies.
It is particularly useful when an experiment requires precise manipulation of individual cells, tissues, organoids, or model organisms. Researchers can use it to introduce a defined material and then assess effects on gene function, tumor development, drug response, or treatment mechanisms. The controlled delivery helps distinguish biological outcomes from variation caused by inconsistent sample handling.
Controlled microinjection can support evaluation of how cells or model systems respond after receiving drugs, nucleic acids, proteins, or tumor cells. Depending on the study, researchers may examine gene function, tumor development, drug response, or treatment mechanisms. Reproducible delivery and reduced sample damage strengthen comparisons among conditions and improve interpretation of observed effects.