Murine Mammary Tumor
Murine Mammary Tumor - Related Videos
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
09:12
Dendra2 Photoswitching through the Mammary Imaging Window
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Cited by 63 •
2009
Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.
03:40
Orthotopic Injection into the Mammary Fat Pad: Establishing Breast Cancer in Mice
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2023
In this article we discuss how to establish a breast cancer model in female mice by injecting breast cancer cells into the mammary fat pad. The example protocol demonstrates the orthotopic injection.
11:31
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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Cited by 5 •
2017
Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.
06:41
Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads
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2023
This video describes a technique of deriving extracellular matrix hydrogels by decellularizing murine mammary fat pads following ex vivo irradiation for in vitro studies. This hydrogel helps to mimic the in vivo breast tissue environment post-radiation treatment to study tumor cell behavior.
FAQs
Accumulated oncogenic changes can disrupt the normal balance between epithelial-cell proliferation, cell death, and tissue organization. As these controls become altered, mammary tissue may progress from regulated growth toward tumor development and further disease advancement. Studying this sequence helps cancer researchers connect specific cellular changes with tumor initiation and progression in a controlled mammalian system.
Spontaneous tumors arise without an externally applied initiating treatment, whereas induced tumors develop after researchers introduce genetic, hormonal, or carcinogen-based influences. This distinction allows investigators to examine different routes to mammary tumor development. Comparing these models can clarify how the initiating factor affects disease emergence, progression, and responses observed during cancer research.
Genetic background and endocrine signals can influence how mammary tumors develop and respond to treatment. These variables provide a way to investigate why similar tumor processes may produce different outcomes in different biological settings. Incorporating them into experimental design helps researchers examine disease development as an interaction between oncogenic changes, host biology, and hormonal context.
These tumors provide controlled systems for examining interactions between cancer cells and the immune microenvironment. Researchers can use that context to study how surrounding biological conditions relate to tumor initiation, progression, metastasis, or treatment response. This perspective extends analysis beyond the tumor cells themselves and supports a broader understanding of cancer as a tissue-level process.
Investigators use these models to examine multiple stages of disease, including tumor initiation, progression, and metastasis. Because the tumors develop in mammary tissue within mice, researchers can evaluate how genetic, hormonal, and environmental factors influence those stages. The resulting comparisons help connect experimental causes with observable patterns of disease development in cancer research.
Murine mammary tumor models support preclinical evaluation by providing controlled systems in which investigators can assess treatment response alongside tumor development. Researchers can examine how responses vary with genetic background, endocrine signals, or environmental influences. These experiments help characterize treatment effects before broader conclusions are considered, while also linking therapeutic outcomes to tumor and host context.