Pediatric Brain Tumors
Pediatric Brain Tumors - Related Videos
09:43
Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
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Cited by 22 •
2017
The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.
07:44
Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device
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2025
The development of orthotopic pediatric brain tumor models requires meticulous precision, using a stereotaxic device to implant cancer cells precisely. The methodology presented here outlines the steps involved in preparing brain tumor cells, performing intracranial injections, and implementing a post-operative monitoring system to assess brain tumor engraftment.
10:13
A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
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Cited by 29 •
2015
Many types of human brain tumors are localized to specific regions within the brain and are difficult to grow in culture. This protocol addresses the role of tumor microenvironment and investigates new drug treatments by analyzing fluorescent primary brain tumor cells growing in an organotypic mouse brain slice.
03:45
Tumor Treating Fields Application in Human Brain Tumor Models: A Non-invasive Technique to Deliver Alternating Electric Fields to Human Brain for Treating Brain Tumor
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2023
This video demonstrates the application of Tumor Treating Fields or TTFields in human brain tumor models. This non-invasive technique delivers low-intensity alternating electric fields directly to the brain, resulting in inhibition of cell division and subsequent apoptosis.
04:27
Co-culturing Glutamatergic Neurons and Pediatric High-Grade Glioma Cells
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2025
This video showcases a technique for co-culturing glutamatergic neurons and pediatric high-grade glioma (pHGG) cells within a microfluidic device. Human induced pluripotent stem cells are introduced into the microfluidic system and incubated to promote their differentiation into mature cortical glutamatergic neurons. Following this, the pHGG cells are introduced onto the mature neurons, establishing a co-culture and enabling interactions between neurons and pHGG cells.
FAQs
Genetic and epigenetic changes can disturb several linked cellular behaviors: proliferation may accelerate, survival controls may be disrupted, and differentiation may be altered. These changes help explain why tumor cells do not follow normal growth regulation. In cancer research, examining both types of change supports more precise classification and can reveal distinctions among tumors that appear similar anatomically.
Developing neural tissue can be affected by the tumor itself as well as by treatment. This creates a research problem that extends beyond controlling tumor growth: investigators must also consider potential effects on normal development and long-term function. Consequently, treatment research emphasizes reducing harm while improving outcomes, rather than focusing only on immediate tumor control.
The skull confines the brain and other central nervous system structures, so expanding tumor growth can increase pressure. A tumor may also damage essential neural pathways, creating consequences linked to location as well as cellular behavior. These mechanical and structural effects are important when researchers interpret neurological findings and assess the potential impact of disease progression.
Evaluation combines several complementary sources of evidence. Neurological assessment examines clinical function, magnetic resonance imaging provides a view of the affected area, tissue analysis examines the tumor directly, and molecular profiling characterizes relevant molecular features. Together, these approaches support diagnosis and help connect the tumor’s observed structure with its biological properties.
Molecular profiling adds information that may not be apparent from imaging or tissue appearance alone. By identifying molecular features associated with a tumor, researchers can refine its classification and use that information when considering drug selection. This approach strengthens the connection between cancer biology and clinical decision-making while supporting more tailored research on pediatric disease.
Researchers combine these findings when they need a more complete basis for treatment planning and outcome improvement. Imaging helps characterize disease in the nervous system, tissue analysis contributes direct tumor evidence, and molecular results refine interpretation. The integrated picture can inform surgery and drug selection while supporting efforts to reduce treatment-related harm in children.