Anesthetized Male Mouse
Anesthetized Male Mouse - Related Videos
02:21
Enriching for Males: Maintaining a Stock of Male C. elegans
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2023
Nematodes can be propagated easily in the lab--in part because hermaphrodites can reproduce by self-fertilization. However, genetic crosses and other experiments may require a large number of males. This video describes a method to enrich for male C. elegans.
04:17
Electrophoretic Delivery of GABA into the Epileptic Focus of an Anesthetized Mouse
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2025
Source: Slezia, A. et al. Electrophoretic Delivery of γ-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice. J. Vis. Exp. (2019)This video demonstrates the electrophoretic delivery of gamma-aminobutyric acid (GABA) to inhibit seizure activity in an anesthetized mouse. It outlines the steps involved in probe implantation, neural activity recording, seizure induction, and controlled GABA delivery via a microfluidic ion pump to assess its therapeutic efficacy.
03:24
Extracting Prostate from Mouse Urogenital System: A Technique to Separate Prostate Gland from Other Urinary and Genital System Organs in Male Mouse
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2023
In this video, we describe the procedure for the dissection of the urogenital system or UGS from a male mouse model. This UGS is further dissected ex vivo to isolate the prostate gland and study its role in prostate cancer development.
10:00
RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
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Cited by 5 •
2020
Here, we describe the immunoprecipitation of ribosomes and associated RNA from select populations of adult male mouse germ cells using the RiboTag system. Strategic breeding and careful immunoprecipitation result in clean, reproducible results that inform on the germ cell translatome and provide insight into the mechanisms of mutant phenotypes.
09:39
Male Rectal Exam
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2023
Source: Joseph Donroe, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT While its usefulness in cancer screening is debated, the male rectal examination remains an important part of the physical exam. The exam is indicated in selected patients with lower urinary tract symptoms, urinary and/or fecal incontinence or retention, back pain, anorectal symptoms, abdominal complaints, trauma patients, unexplained anemia, weight loss, or bone pain. There are no absolute...
FAQs
Anesthetic agents depress central nervous system activity, producing reduced awareness and limiting movement during cancer procedures. These effects help investigators perform implantation, imaging, biopsy, surgery, drug administration, or tissue collection under more controlled conditions. Because anesthesia also coincides with changes that must be managed in respiration, circulation, and body temperature, physiological monitoring remains essential for interpreting experimental results.
Monitoring these functions helps maintain the physiological conditions required for a controlled experiment. Anesthesia can influence central nervous system activity, so researchers must pay attention to respiration, circulation, and temperature while the mouse undergoes a procedure. Maintaining these conditions supports consistent observations and helps distinguish findings related to the cancer model from variation associated with the experimental state.
Male mice provide a defined biological context for examining sex-specific influences on tumor growth, treatment response, and disease mechanisms. Their use does not replace studies in other biological contexts; instead, it allows researchers to evaluate whether cancer-related outcomes differ by sex. This consideration is especially relevant when interpreting tumor behavior or responses to an intervention.
Reduced movement limits motion-related variability during procedures such as imaging, biopsy, surgery, and tissue collection. A controlled anesthetized state can therefore make observations and sample collection more standardized across animals. Consistency still depends on maintaining physiological conditions and applying appropriate animal-welfare practices, including attention to the mouse during the procedure and recovery.
The model can support several stages of cancer investigation, including tumor implantation, imaging, biopsy, surgery, drug administration, and tissue collection. The specific procedure determines what information or material becomes available for analysis. Using anesthesia allows these activities to be conducted while limiting movement and reducing awareness or pain, with monitoring used to maintain physiological stability.
Researchers would choose it when a cancer procedure requires immobility, reduced awareness, or minimized pain, particularly for interventions or measurements that movement could disrupt. It is relevant to studies involving tumors, treatment administration, imaging, surgical manipulation, or collection of biological material. The male biological context also supports questions about sex-specific tumor and treatment responses.
Studies can examine tumor growth, treatment response, and disease mechanisms while using a defined male-mouse context. Depending on the procedure, investigators may obtain imaging observations, biopsy material, surgical findings, or collected tissues. Interpreting these outcomes requires attention to anesthesia-related physiological conditions and recovery, because controlled monitoring helps preserve the reliability and welfare of the experiment.