Jagged1-fc
Jagged1-fc - Related Videos
03:03
Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies
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2025
This video demonstrates an assay measuring the antibody-mediated activation of T cell effector functions. Adding an influenza virus hemagglutinin (HA)-specific monoclonal antibody to transfected mammalian cells expressing HA results in the formation of immune complexes with HA. Upon introducing engineered T cells expressing Fc receptors and a luciferase reporter, the T cells are activated by the antibody-HA complexes, and this activation is detected by adding a luciferase substrate and...
10:33
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Cited by 3 •
2014
Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.
04:47
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
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Cited by 6 •
2018
We describe a method to measure the activation of Fc-mediated effector functions by antibodies that target the influenza virus hemagglutinin. This assay can also be adapted to assess the ability of monoclonal antibodies or polyclonal sera targeting other viral surface glycoproteins to induce Fc-mediated immunity.
03:32
An In Vitro Artificial Activation Assay for Studying Fc Receptor Activation by Antibodies
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2025
This video demonstrates FcγRIIIa-driven events initiated by therapeutic antibodies in human natural killer cells. The artificial stimulation platform facilitates the exploration of downstream effector functions, encompassing cytoskeletal rearrangement, degranulation, chemokine/cytokine production, and signaling pathways mediated by the FcγRIIIa and Fc portions of antibodies involved in binding.
08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Cited by 13 •
2017
Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.
FAQs
The Fc portion gives Jagged1-Fc an antibody-derived domain that supports a defined fusion format for presenting Jagged1 in culture. This matters because researchers can use the same recombinant ligand as a controlled stimulus rather than relying on an unspecified cellular source. In developmental experiments, that defined presentation helps isolate how Jagged1-Notch engagement affects cell behavior.
Surface immobilization places the Jagged1 component on a culture substrate so it can engage Notch receptors on neighboring cells. This receptor-ligand interaction promotes cleavage of the receptor, releasing the Notch intracellular domain. The intracellular domain then enters the nucleus, where it regulates target gene expression, linking the experimental surface cue to a transcriptional response.
Activation is transmitted through a sequence from receptor engagement to receptor cleavage, release of the Notch intracellular domain, and its movement into the nucleus. Nuclear regulation of target genes can alter developmental decisions, including differentiation and cell fate. Jagged1-Fc therefore helps researchers examine how a defined signaling input contributes to tissue patterning and developmental behavior.
A supported workflow is to immobilize Jagged1-Fc on a culture surface, place cells in contact with that presentation, and examine the resulting Notch pathway activity. Readouts can include changes in target gene expression and associated cellular behavior. The same strategy can be adapted to organoid models when investigators need to study signaling during tissue organization.
Researchers choose this reagent when they need controlled, defined Jagged1 presentation to manipulate Notch signaling in cultured cells or organoids. It is particularly useful for testing how ligand-receptor interactions influence cell fate, differentiation, or tissue patterning. Because the input is experimentally supplied, observed changes can be related to the Jagged1-mediated signaling condition used in the model.
These experiments can connect a Notch signaling input with downstream gene regulation and developmental behavior. In cell cultures and organoids, researchers can investigate whether Jagged1-mediated signaling is associated with altered differentiation, cell fate, tissue patterning, or disease-related cellular behavior. Such models link a defined ligand presentation to outcomes that emerge at cellular or tissue scale.