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A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

DOI:
10.3791/56256-v

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04:47 min

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February 23, 2018

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, , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease,J. Craig Venter Institute

Chapters

  • 00:05Title
  • 00:42Influenza Virus Hemagglutinin Expression
  • 02:18Antibody-dependent Cell-mediated Cytotoxicity (ADCC) Analysis
  • 04:01Results: Representative ADCC Analysis
  • 04:24Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method to measure the activation of Fc-mediated effector functions by antibodies that target the influenza virus hemagglutinin. This assay can also be adapted to assess the ability of monoclonal antibodies or polyclonal sera targeting other viral surface glycoproteins to induce Fc-mediated immunity.

Tags

Keywords: Fc-mediated Effector FunctionsInfluenza HemagglutininAntibodyADCCCell-mediated CytotoxicityTransfectionInfectionA549 Cells293 CellsVirusImmunologyVaccinology

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