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1Department of Orthopaedic Surgery,Perelman School of Medicine, University of Pennsylvania, 2Department of Biological Sciences,University of the Sciences, 3The Department of Small Animal Clinical Sciences,College of Veterinary Medicine, Michigan State University, 4Department of Biology,College of Science, Technology, Engineering, & Mathematics, Eastern Washington University
Chapters
- 00:05Title
- 00:51Bone Marrow Cell Isolation
- 02:45Osteoclast Differentiation
- 03:55Jagged1-coated Surface Continuous and Temperary Notch Signaling Stimulation
- 06:08Results: Representative Effects of Jagged1-Fc Signaling on Osteoclasts Precursor Gene Expression
- 07:24Conclusion
Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.
