Mouse Embryo Gastrulation
Mouse Embryo Gastrulation - Related Videos
09:14
Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation
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Cited by 4 •
2024
This paper establishes a pipeline for high-quality single-cell and nuclei suspensions of gastrulating mouse embryos for sequencing of single cells and nuclei.
01:56
Gastrulation
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2025
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
09:36
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
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Cited by 10 •
2018
The node and notochordal plate are transient signaling organizers in developing mouse embryos that can be visualized using several techniques. Here, we describe in detail how to perform two of the techniques to study their structure and morphogenesis: 1) scanning electron microscopy (SEM); and 2) whole mount immunofluorescence (WMIF).
02:52
Prenatal Mouse Embryos Retrieval: A Procedure to Harvest Embryos from Pregnant Mouse
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2025
This video demonstrates the retrieval of embryos from a pregnant mouse. The recovered perinatal embryos can be used as model systems for studying mammalian development.
09:57
Dissection of 6.5 dpc Mouse Embryos
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Cited by 28 •
2007
Isolation of postimplantation-stage embryos allows one to study gene patterning and analyze cell-lineage decision making processes during embryonic development, but proper dissection of the early embryo can be challenging. This protocol describes a method for isolating early primitive-streak-stage embryos (~6.5 days post coitum [dpc]).
FAQs
Epithelial-to-mesenchymal transition allows epiblast cells associated with the primitive streak to change their behavior and migrate inward. This movement is essential for placing cells beneath the remaining epiblast, where they contribute to definitive endoderm and mesoderm. Studying this transition helps explain how coordinated cell behavior converts an initially simple embryonic arrangement into organized tissues.
The primitive streak serves as the region toward which epiblast cells converge before internalizing and migrating. Its activity coordinates the entry of cells that will form definitive endoderm and mesoderm, while epiblast cells that remain outside this process contribute to ectoderm. Consequently, streak-associated movements link cell position with early lineage specification.
Signaling events help coordinate the cell movements and lineage decisions that occur during gastrulation. Their effects are relevant to tissue patterning as well as anterior-posterior and left-right organization, so they connect local cellular behavior with the larger body plan. Investigating these signals can therefore clarify how early positional information is established before organ formation.
Lineage specification emerges as epiblast cells follow different developmental paths during their movement through the primitive streak. Internalizing cells contribute to definitive endoderm or mesoderm, whereas cells that remain in the epiblast become ectoderm. This relationship makes gastrulation useful for examining how changing position and coordinated migration are associated with the first major tissue identities.
Studies commonly focus on coordinated cell movements, primitive-streak activity, epithelial-to-mesenchymal transition, signaling events, and the resulting tissue patterning. These observations can be used to relate cellular behavior to germ-layer formation, body-axis organization, and early lineage specification. The combined view is more informative than examining cell migration or tissue identity in isolation.
Mouse gastrulation provides a mammalian context for investigating how early embryos establish tissues and body organization. Findings can support interpretation of congenital defects and can be compared with results from stem cell and embryo models. Such comparisons help developmental biologists assess which principles of cell movement, signaling, and patterning are shared across experimental systems.
Because gastrulation establishes the germ layers and organizes anterior-posterior and left-right patterning, disrupted cellular movements or signaling during this stage may help explain later developmental abnormalities. Mouse studies allow researchers to connect early patterning events with congenital defects and to interpret those findings alongside evidence from stem cell and embryo models.