Pre-implantation Development
Pre-implantation Development - Related Videos
07:34
FISH for Pre-implantation Genetic Diagnosis
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Cited by 17 •
2011
This article describes the selection of suitable probes for single-cell FISH, spreading techniques for blastomere nuclei, and in situ hybridization and signal scoring, applied to pre-implantation genetic diagnosis (PGD) in a clinical setting.
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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Cited by 4 •
2017
Microarray technology allows quantitative measurement and gene expression profiling of transcripts on a genome-wide basis. Therefore, this protocol provides an optimized technical procedure in a two-color custom made bovine array using Day 7 bovine embryos to demonstrate the feasibility of using low amount of total RNA.
09:30
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
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Cited by 3 •
2022
The protocol presents the overall in-lab procedures required in pre-implantation genetic testing for aneuploidy on a semiconductor-based next-generation sequencing platform. Here we present the detailed steps of whole genome amplification, DNA fragment selection, library construction, template preparation, and sequencing working flow with representative results.
12:09
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
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Cited by 6 •
2022
A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.
03:49
Generating Renal Cell Carcinoma Model: A Protocol to Develop Orthotopic RCC Murine Model by Intrarenal Implantation of Cancer Cells
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2023
In this video, we develop an orthotopic mouse model of renal cell carcinoma. This model results in reproducible primary tumors in the injected kidney and metastatic tumors in the lung, allowing the evaluation of disease progression and treatment.
FAQs
Cleavage divisions increase the number of embryonic cells while keeping overall embryo size relatively stable. This arrangement allows early cell production to proceed without immediate expansion of the embryo, creating the cellular basis for later organization. In developmental biology, distinguishing cell-number increase from growth helps researchers interpret how early embryos progress toward compaction and subsequent specialization.
Compaction marks a change from relatively separate cells to a more organized morula. This transition links early cleavage with the first visible organization of the embryo and occurs before blastocyst formation. Studying compaction therefore helps explain how coordinated cellular behavior precedes lineage separation and prepares the developing embryo for further specialization before implantation.
Blastocyst formation establishes two distinct cellular populations: the inner cell mass and the trophectoderm. Their appearance provides an early example of cellular specialization rather than merely an increase in cell number. This distinction is important in developmental biology because it connects early embryonic organization with the emerging body plan and preparation for attachment to the uterine lining.
Activation of developmental gene networks is one outcome examined during pre-implantation development. These networks provide a molecular context for establishing the embryo’s early body plan, complementing observations of cleavage, compaction, and lineage formation. Studying the stage at both cellular and gene-regulatory levels helps connect visible structural changes with the underlying organization of early embryogenesis.
A basic study framework follows the embryo from fertilization through cleavage, compaction, morula formation, and blastocyst formation. Researchers can then relate each morphological transition to cell specialization and the emergence of the inner cell mass and trophectoderm. This staged approach organizes observations around developmental progression rather than treating early embryogenesis as a single event.
Embryo culture provides a research setting for examining pre-implantation changes before uterine attachment. Within this context, investigators can study divisions, cellular organization, specialization, and blastocyst formation. These observations support developmental-biology research while connecting laboratory investigation with questions about embryo progression, preparation for implantation, and the causes of early developmental failure.
In vitro fertilization research uses knowledge of this interval to focus attention on embryo behavior before implantation. The same developmental framework supports infertility studies by examining how early progression relates to preparation for uterine attachment. It also helps investigate early developmental failure, making these stages relevant to both basic developmental biology and reproductive research.