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Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

DOI:
10.3791/63388-v

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12:09 min

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August 10, 2022

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, , , , , , ,
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA),Vienna Biocenter (VBC), 2Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), 3Vienna BioCenter PhD Program,Doctoral School of the University of Vienna and Medical University of Vienna

Chapters

  • 00:04Introduction
  • 01:24Formation of Naive PSC Aggregates
  • 04:57Blastoid Development
  • 05:49Formation of Blastoids in 96-well Ultra-low Attachment Microplates
  • 07:05Formation of Trophospheres
  • 08:05Results: Blastoid and Trophospheres Derived from Aggregates of Naïve hPSC
  • 11:20Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.

Tags

Keywords: Human BlastoidsBlastocyst DevelopmentImplantationIn Vitro FertilizationPre-clinical ModelingTherapeutic TargetsNon-hormonal ContraceptivesMEF ExclusionPXGL MediaN2B27 Basal MediaAggregation MediaCell DetachmentMicroWells

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