Replica Plating Strategy
Replica Plating Strategy - Related Videos
09:23
Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures
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Cited by 7 •
2012
We present methods for fabrication of patterned microstructures of vertically aligned carbon nanotubes (CNTs), and their use as master molds for production of polymer microstructures with organized nanoscale surface texture. The CNT forests are densified by condensation of solvent onto the substrate, which significantly increases their packing density and enables self-directed formation of 3D shapes.
06:53
A Rat Tibial Growth Plate Injury Model to Characterize Repair Mechanisms and Evaluate Growth Plate Regeneration Strategies
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Cited by 22 •
2017
The growth plate is a cartilaginous region in children's long bones where longitudinal growth occurs. When injured, bony tissue can form and impair growth. We describe a rat model of growth plate injury that leads to bony repair tissue, allowing the study of repair mechanisms and growth plate regeneration strategies.
09:49
Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
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Cited by 6 •
2016
This article illustrates how the expression of neurotransmitter receptors can be quantified and the pattern analyzed at synapses with identified pre and postsynaptic elements using a combination of viral transduction of optogenetic tools and the freeze-fracture replica immunolabeling technique.
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
09:45
New Variations for Strategy Set-shifting in the Rat
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Cited by 3 •
2017
Set-shifting, a form of behavioral flexibility, requires an attentional shift from one stimulus dimension to another. We extended an established rodent set-shifting task1 by requiring attention to different stimuli according to context. The task was combined with specific lesions to identify neuron subtypes underlying a successful shift.
FAQs
Maintaining each colony’s position creates a direct correspondence among the original culture and every recipient plate. Researchers can therefore determine which colony produced a particular growth pattern without mixing candidates or losing track of their source. This spatial reference is especially valuable when one condition permits growth while another exposes a mutant phenotype.
Growth is compared across recipient plates containing different selective or environmental conditions. A colony that grows on one medium but fails on another becomes a candidate for an auxotrophic or environmentally sensitive phenotype. The comparison is more informative than observing a single plate because it connects the failure pattern to the specific condition that challenged the cells.
The sterile velvet or membrane serves as the transfer surface between plates. It picks up cells from the established colony pattern and deposits them onto fresh agar while retaining the original spatial arrangement. This mechanism allows multiple conditions to be tested from one source plate and reduces the need to recreate each colony separately.
Researchers first establish colonies on an original agar plate, then use a sterile velvet or membrane to collect cells in their existing pattern. The transfer surface is applied to multiple fresh media, including selective conditions, and the resulting growth patterns are compared with the original. The source plate remains available for analyzing colonies identified by the screen.
In developmental biology, replica plating supports genetic screens in microbial models when researchers need to identify mutants with altered differentiation, morphogenesis, or developmental responses. Comparing colony growth under different conditions can highlight strains whose developmental phenotypes depend on particular environmental or nutritional settings, helping prioritize candidate genes for further study.
The pattern of growth and growth failure across replicate plates provides a practical link between a colony’s genetic state and its observed phenotype. Researchers can identify candidate auxotrophic or environmentally sensitive mutants, then return to the preserved original colony for further analysis. In developmental screens, these candidates may point toward genes affecting differentiation, morphogenesis, or developmental responses.