Phospholipid Coating
Phospholipid Coating - Related Videos
09:52
A Fluorescence-based Assay of Phospholipid Scramblase Activity
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Cited by 61 •
2016
We describe a fluorescence-based assay to measure phospholipid scrambling in large unilamellar liposomes reconstituted with opsin.
07:54
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
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Cited by 2 •
2015
We present a new technique to measure the lateral diffusion of a surface active species at the fluid-fluid interface by merging a droplet monolayer onto a flat monolayer.
02:43
Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions
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2025
In this video, we demonstrate the interaction between proteins and artificial phospholipid vesicles using quantitative flow cytometry. The binding of fluorescently-labeled proteins to fluorescently-labeled phospholipid vesicles increases the mean fluorescence intensity, and this increase is detected by a flow cytometer.
10:03
Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
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Cited by 133 •
2016
Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.
07:08
High-Density Lipoprotein-Specific Phospholipid Efflux Assay
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2025
Herein, we describe the protocols to measure the amount of fluorescent phospholipid specifically removed from lipid donor particles by high-density lipoprotein (HDL) present in whole human plasma or serum. We have shown that this metric of HDL functionality predicts incident cardiovascular disease.
FAQs
Water associated with the phospholipid heads forms a hydration layer at the engineered interface. This water-rich region can make nonspecific adsorption less favorable and reduce interfacial friction. As a result, the coated material may interact more selectively with proteins, cells, or surrounding fluids than an unmodified surface, which is important when engineers seek improved compatibility.
The amphiphilic arrangement of the molecules controls the resulting architecture in aqueous conditions. Hydrophilic heads orient toward surrounding water, while hydrophobic tails associate with one another, supporting either a monolayer or bilayer. Engineers can therefore select a coating configuration suited to the desired interface, such as a device surface, nanoparticle, or sensor.
Phospholipid chemistry provides a way to tune how the interface interacts with its environment. Changes in the coating design can influence protein and cell interactions, surface compatibility, and the stability of the engineered interface. This tunability helps connect a coating choice to a specific engineering goal rather than treating every application as requiring the same surface.
An unmodified surface does not intentionally provide the organized, water-rich interface created by phospholipids. Coating adds a designed interfacial layer that can reduce nonspecific adsorption and friction while influencing protein and cell interactions. This distinction matters in engineering because surface behavior, rather than only the bulk material, can determine compatibility and performance in aqueous or biological settings.
Engineers should match the coating design to the material's intended environment and function. Relevant considerations include whether the application needs reduced nonspecific adsorption, lower interfacial friction, improved biocompatibility, controlled protein or cell interactions, or interface stabilization. The available chemical tunability also supports designs tailored to devices, nanoparticles, sensors, and delivery systems.
Phospholipid coatings are relevant to medical devices, nanoparticles, sensors, and other engineered materials. In these settings, the coating can support biocompatibility, regulate interactions with proteins and cells, and stabilize interfaces. The same surface-engineering approach also contributes to targeted delivery systems and advanced nanotechnology designs where controlled biological interactions are important.
For nanoparticles, a phospholipid interface offers a tunable surface for controlling interactions with proteins and cells. That control can be incorporated into delivery-system designs intended to improve compatibility or guide biological interactions. In biomedical engineering, the coating therefore serves not only as a protective interface but also as a design element for more specialized nanoparticle behavior.