Syringe Flow
Syringe Flow - Related Videos
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
0 Views •
2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
0 Views •
Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
0 Views •
Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
05:49
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
0 Views •
Cited by 2 •
2021
Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
0 Views •
Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
FAQs
Higher fluid viscosity and greater resistance at the needle or connected channel increase the pressure required to maintain a given flow. Because narrow outlets create substantial pressure losses, small changes in outlet geometry can strongly affect delivery behavior. Engineers therefore evaluate fluid properties and outlet resistance together when predicting flow rate and selecting operating conditions.
Plunger speed controls how quickly the syringe displaces liquid, making it a primary factor in flow-rate control. Faster advancement generally drives more rapid transport, while changes in fluid resistance determine how much pressure is needed. Adjusting displacement speed also helps engineers examine transient behavior, including how the system responds when operating conditions change.
Outlet geometry governs the resistance encountered as liquid leaves the barrel. A narrow needle or channel produces greater resistance and pressure loss than a less restrictive outlet, so the same plunger motion can produce different delivery behavior in different configurations. Accounting for outlet dimensions is essential when comparing systems or designing controlled fluid transport.
Calibration begins by relating controlled plunger displacement or motion to the resulting flow behavior under defined fluid and outlet conditions. Engineers can then compare the expected and observed transport response, identify pressure-loss or resistance effects, and adjust the operating relationship used by the system. This supports more precise and repeatable delivery of small liquid volumes.
These systems are useful when engineers need controlled, repeatable transport of small fluid volumes. Syringe-flow analysis supports syringe-pump design and helps characterize delivery through microfluidic channels, where outlet resistance and fluid properties influence performance. The same principles also apply to dosing systems and material-deposition processes that require carefully controlled liquid movement.
Analysis can support prediction of transient behavior, calibration of delivery systems, and optimization of operating conditions. By examining plunger displacement speed, viscosity, pressure losses, and outlet resistance together, engineers can identify how design or control changes affect transport. The resulting understanding helps improve dosing consistency, material deposition, and repeatability in small-volume fluid systems.