Automated Isolation
Automated Isolation - Related Videos
08:06
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
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Cited by 1 •
2016
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
13:49
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
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Cited by 6 •
2017
Biopanning bacterial display libraries is a proven technique for discovery of peptide affinity reagents, a robust alternative to antibodies. The semi-automated sorting method herein has streamlined biopanning to decrease the occurrence of false positives. Here we illustrate the thought process and techniques applied in evaluating candidates and minimizing downstream analysis.
10:55
Digital Microfluidics for Automated Proteomic Processing
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Cited by 30 •
2009
Digital Microfluidics is a technique characterized by the manipulation of discrete droplets (~nL - mL) on an array of electrodes by the application of electrical fields. It is well-suited for carrying out rapid, sequential, miniaturized automated biochemical assays. Here, we report a platform capable of automating several proteomic processing steps.
08:35
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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Cited by 53 •
2015
The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.
01:17
Marketing Automation
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2025
In today's fast-paced digital landscape, businesses are constantly seeking ways to streamline operations, improve customer engagement, and ultimately drive growth. One of the most transformative tools in this endeavor is marketing automation. Marketing automation refers to the use of software and technology to automate various marketing tasks and workflows, such as email marketing, social media management, lead generation, and customer segmentation. By automating these repetitive tasks,...
FAQs
These capture materials provide the separation surface within an automated workflow. After sample lysis, genetic material is retained on magnetic particles or a specialized membrane, while washing removes unwanted contaminants. Elution then transfers the purified DNA or RNA into a clean solution. The capture and release stages make the preparation suitable for later genetic analysis.
Each stage contributes a different requirement for usable genetic material. Lysis prepares the biological sample, capture retains nucleic acids, washing removes contaminants, and elution releases the material into a clean solution. Automated control of volumes and timing helps coordinate these conditions consistently, which supports reliable preparation for downstream genotyping, sequencing, or gene expression studies.
Robotic systems apply programmed volumes, timing, and handling across samples rather than relying entirely on manual execution. This standardization reduces differences introduced during processing and limits unnecessary sample handling. Consistent operation is especially valuable when many samples must be prepared for comparison, because processing variability and contamination risks can otherwise affect downstream genetic results.
A typical workflow begins with sample lysis, followed by nucleic-acid capture on magnetic particles or a specialized membrane. The system then performs washing steps to remove contaminants and elutes the retained material into a clean solution. Throughout the procedure, the instrument manages volumes and timing while maintaining sample tracking, creating prepared DNA or RNA for subsequent analysis.
The workflow requires an instrument capable of coordinating robotic sample processing, along with a capture medium such as magnetic particles or a specialized membrane. Samples also pass through lysis, washing, and elution stages, with the final material collected in a clean solution. Sample tracking is an additional operational component that helps maintain identity and workflow consistency.
Automated isolation is useful when genetics workflows require consistent preparation across multiple biological samples. The resulting DNA or RNA can support genotyping, sequencing, and gene expression studies, as well as diagnostic research. Its ability to increase throughput while reducing manual handling makes it relevant when sample numbers, processing consistency, and contamination control are important.