Hybrid Capture Enrichment
Hybrid Capture Enrichment - Related Videos
10:24
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
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Cited by 23 •
2015
The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
13:32
Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
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Cited by 22 •
2013
We describe how to use laser capture microdissection (LCM) to obtain enriched populations of hippocampal neurons or single neurons from frozen sections of the injured rat brain for subsequent gene expression analysis using quantitative real time PCR and/or whole-genome microarrays.
09:27
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
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Cited by 2 •
2018
This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.
10:11
Glycopeptide Capture for Cell Surface Proteomics
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Cited by 8 •
2014
Cell surface proteins are biologically important and widely glycosylated. We introduce here a glycopeptide-capture approach to solubilize, enrich, and deglycosylate these proteins for facile LC-MS based proteomic analyses.
10:44
Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
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Cited by 1 •
2025
Here, we present a protocol to enable whole-genome sequencing of bacterial sexually transmitted infections from clinical samples using target enrichment. This novel, syndromic panel-based method overcomes challenges of abundant human DNA and low bacterial loads, facilitating genomic surveillance for Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, and Mycoplasma genitalium.
FAQs
Probe design determines which loci are preferentially recovered. Each labeled oligonucleotide is selected to complement a genomic region of interest, so sequence complementarity allows a probe-target hybrid to form. Regions lacking a matching target are less likely to be retained during enrichment. This selectivity lets sequencing focus on chosen genes or exonic regions rather than the entire genomic library.
Affinity-based beads provide the physical separation step. After probe-target hybrids form, the beads isolate those labeled complexes from the broader DNA library, while much of the non-target material remains outside the enriched fraction. This separation is important because enrichment is not achieved by probe binding alone; recovery of bead-associated material creates a sequencing input with a higher proportion of relevant loci.
Hybrid capture enrichment improves analysis efficiency by reducing off-target reads and increasing coverage of selected regions. More reads directed toward relevant loci can make sequence information easier to examine across genes or exons and support detection of variants within those targets. The benefit is concentrated information about regions of interest, rather than simply generating a larger amount of sequencing data.
A typical workflow begins with a genomic DNA library and probes designed for selected regions. The library is exposed to labeled probes so complementary targets can hybridize, and affinity-based beads then isolate the resulting complexes. The enriched material proceeds to sequencing, where reads are evaluated for variation in the targeted loci. Each stage connects selective capture with the final genetic analysis.
The choice follows the scope of the genetic question. Whole-exome sequencing concentrates analysis across exonic regions, whereas a targeted gene panel focuses enrichment on selected genes or loci. Both approaches use the same capture principle, but their target breadth differs, affecting which variants can be investigated. Researchers can therefore align enrichment design with either broader exonic analysis or a narrower gene-focused study.
Sequencing the captured material supports identification of mutations and characterization of genetic variation across selected loci. In genetics research, this makes the method useful for examining disease-associated genes, provided those genes or regions are included among the capture targets. Results therefore reflect both the DNA sample and the design of the probe set, which determines the genomic territory available for analysis.