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Glycopeptide Capture for Cell Surface Proteomics
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JoVE Journal Biology
Glycopeptide Capture for Cell Surface Proteomics

Glycopeptide Capture for Cell Surface Proteomics

DOI:
10.3791/51349-v

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10:11 min

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May 09, 2014

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,
1Department of Chemistry,Simon Fraser University

Chapters

  • 00:05Title
  • 01:37Harvest Membranes
  • 02:54Dissolve, Denature, and Digest Membrane Proteins
  • 05:34Glycopeptide Capture
  • 08:04Results: LC-MS Based Proteomic Analysis of Cell Surface Proteins
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Cell surface proteins are biologically important and widely glycosylated. We introduce here a glycopeptide-capture approach to solubilize, enrich, and deglycosylate these proteins for facile LC-MS based proteomic analyses.

Tags

Keywords: Glycopeptide CaptureCell Surface ProteomicsN-glycoproteomeSurface ProteinsBiomarker DiscoveryCell-type IdentificationDrug-target SelectionCellular BiologyCellular FunctionsPost-translational ModificationsLC-MSCancerStem CellsDrug Toxicity

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