Ms2-tagged Rna
Ms2-tagged Rna - Related Videos
02:30
Isolation of Small Regulatory RNA–Bound Bacterial Target RNA Using Affinity Purification
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2025
Source: Mercier, N., et. al., MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria. J. Vis. Exp. (2021)This video demonstrates the isolation of MS2-tagged small regulatory RNA (sRNA) bound to its bacterial target RNA using affinity purification with a maltose-binding protein (MBP)–MS2 coat protein fusion immobilized on amylose resin.
08:34
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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Cited by 10 •
2021
MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive...
11:19
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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2016
RNA-protein interactions lie at the heart of many cellular processes. Here, we describe an in vivo method to isolate specific RNA and identify novel proteins that are associated with it. This could shed new light on how RNAs are regulated in the cell.
06:06
Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
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Cited by 5 •
2019
Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.
09:54
RNA-Seq
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2023
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown transcripts.
FAQs
Repeated MS2 stem-loops provide multiple binding sites for the MS2 coat protein. When that coat protein carries a fluorescent protein, the assembled complexes generate a detectable signal associated with the engineered transcript. This arrangement lets researchers follow the labeled RNA without directly staining it, while preserving the system’s focus on transcript-specific behavior.
The fused coat protein connects the genetic label to fluorescence. It recognizes the MS2 stem-loop sequences inserted into the target transcript, and its fluorescent component makes the bound RNA detectable in living cells. Consequently, the observed signal can be used to examine where the transcript is located and how its position changes over time.
MS2-tagged RNA experiments can track several related behaviors rather than providing only a fixed location. Researchers can examine transcript localization, transport, abundance, and movement over time. Considering these features together helps connect RNA distribution and dynamics with cellular organization, and it supports analysis of how gene expression is regulated after transcription.
The target transcript is genetically engineered to contain repeated MS2 bacteriophage stem-loop sequences. The experiment also uses MS2 coat protein fused to a fluorescent protein so the coat protein can bind the inserted sequences and generate a signal. In living cells, this paired design enables observation of the selected RNA and its behavior over time.
The method is especially useful when researchers need to connect RNA location with movement or changing cellular organization. Because the labeled transcript can be observed in living cells over time, experiments can follow transport and dynamic redistribution rather than relying only on a fixed snapshot. This makes the approach relevant to studies of RNA dynamics and post-transcriptional regulation.
This system supports investigations of transcription, post-transcriptional regulation, and the behavior of RNA within cells. Researchers can use it to relate gene expression to cellular organization and to examine how RNA dynamics contribute to broader processes. The overview identifies applications spanning development, disease, and cellular function, making the method relevant across several biological contexts.