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JoVE Journal
Developmental Biology
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Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
 

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Article DOI: 10.3791/52654
January 31st, 2015

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Summary January 31st, 2015

We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.

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