Chemokine Production
Chemokine Production - Related Videos
10:56
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
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Cited by 7 •
2013
The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
06:48
Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses
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Cited by 1 •
2020
Here we describe protocols to perform live imaging and quantitative analysis of chemoattractant receptor dynamics in zebrafish...
06:56
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
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Cited by 22 •
2018
A flow cytometry-based cellular binding assay is described that is primarily used as a screening tool to identify compounds that inhibit the binding of a fluorescently labeled CXC chemokine ligand 12 (CXCL12) to the CXC chemokine receptor 4 (CXCR4).
10:02
An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
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2019
To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration.
10:03
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
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Cited by 4 •
2014
Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.
FAQs
Microbial products, inflammatory cytokines, and tissue injury activate transcriptional programs in immune and stromal cells. These programs increase the synthesis and release of particular chemokines in response to local conditions. Because different triggers can act on different cell populations, the resulting signals help coordinate immune-cell recruitment according to the needs of an infected, inflamed, or repairing tissue.
A concentration gradient provides directional information rather than merely signaling that inflammation is present. Chemokines released from affected tissues create varying concentrations across the surrounding environment, and leukocytes respond through specific G protein-coupled receptors. This arrangement guides cells toward sites where chemokine signals are strongest, helping organize their movement during infection, inflammation, and tissue repair.
G protein-coupled receptors provide the recognition step that allows leukocytes to respond to chemokines. Binding between a chemokine and its compatible receptor converts an extracellular concentration pattern into a migration response. Receptor specificity therefore helps determine which immune cells can follow a given signal, making receptor engagement central to selective recruitment and coordinated inflammatory responses.
Both immune and stromal cells can activate transcriptional programs that produce chemokines, but their contributions occur within different cellular contexts. Immune cells may participate directly in inflammatory signaling, while stromal cells can contribute signals from the surrounding tissue environment. Studying these sources helps researchers determine how local tissues and immune populations jointly shape leukocyte recruitment.
Chemokine-production assays measure how cells respond to relevant stimuli by examining the production or release of chemokines. Researchers can use these assays to investigate responses to microbial products, inflammatory cytokines, or tissue injury-related signals. The resulting measurements help connect cellular activation with immune-cell recruitment and provide experimental evidence about how inflammation is coordinated.
In infectious-disease research, examining chemokine production helps clarify how infected tissues recruit leukocytes and organize local immune responses. Investigators can relate triggering conditions to chemokine release and then consider how receptor-mediated migration shapes cell accumulation at affected sites. This information supports analysis of host responses to infection and the mechanisms coordinating inflammation in tissues.
Chemokine production is relevant because altered cell-trafficking signals can influence where immune cells accumulate within tissues. Research in autoimmune disorders and cancer can examine these signals to understand harmful inflammation or immune-cell recruitment in diseased environments. Chemokine-production studies also support therapeutic strategies designed to modify cell trafficking or limit inflammatory effects when recruitment becomes damaging.