Clp Mouse Model
Clp Mouse Model - Related Videos
09:17
Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness
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Cited by 18 •
2017
This protocol describes a centrally catheterized mouse model of prolonged critical illness. We combine the cecal ligation and puncture method to induce sepsis with the use of a central venous line for fluids, drugs and nutrient administration to mimic the human clinical setting.
07:34
Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies
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Cited by 13 •
2012
Sepsis refers to a systemic inflammatory response syndrome resulting from a microbial infection, and can be simulated by a surgical technique termed cecal ligation and puncture (CLP). Here we describe a method to use CLP-induced animal model to screen medicinal herbs for therapeutic agents.
06:24
Mouse Models of Periventricular Leukomalacia
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Cited by 28 •
2010
We established mouse models of periventricular leukomalacia (PVL), the predominant brain injury in premature infants characterized by periventricular white matter lesions. Hypoxia/ischemia with/without systemic infection are the primary causes of PVL. Unilateral carotid ligation and hypoxia exposure with/without lipopolysaccharide injection creates PVL-like lesions in P6 mice.
10:42
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
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Cited by 16 •
2012
NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
FAQs
Puncturing the ligated cecum releases intestinal contents into the peritoneal cavity, creating a severe abdominal infection rather than exposure to a single organism. This polymicrobial challenge drives local inflammation, tissue injury, and broader immune activation. The resulting progression allows investigators to examine how host defenses respond when infection extends beyond its original abdominal site.
Ligation length and puncture size are key variables for adjusting the intensity of disease. Changes in these features alter the amount or extent of intestinal-content release, which can influence infection, inflammation, tissue injury, and systemic effects. Researchers use this flexibility to produce different levels of severity when studying sepsis mechanisms or treatment responses.
The model supports analysis of both protective and harmful immune responses during severe infection. Investigators can examine host defense against polymicrobial disease, cytokine production, and the development of immunosuppression after systemic activation. This makes the approach useful for studying how an initially intense response may coexist with impaired immunity during sepsis.
Survival, cytokine production, bacterial dissemination, and organ dysfunction provide complementary views of disease progression. Survival reflects overall outcome, while cytokines indicate immune activation, bacterial dissemination shows whether infection has spread, and organ dysfunction captures tissue-level consequences. Considering these measures together helps distinguish changes in infection, inflammation, and systemic injury.
The core procedure requires ligating the cecum and creating a puncture that permits intestinal contents to enter the peritoneal cavity. Researchers pay particular attention to ligation length and puncture size because these features affect disease severity. Subsequent evaluation includes monitoring survival, immune signals, bacterial spread, and organ dysfunction to characterize the induced illness.
This in vivo model is especially valuable when researchers need to study sepsis arising from severe abdominal infection and a polymicrobial challenge. It can support investigations of sepsis pathogenesis, host defense, and immunosuppression, while also providing a preclinical setting for evaluating antimicrobial or immune-modulating treatments through measurable disease and immune outcomes.